Induction of programmed cell death by Prangos uloptera, a medicinal plant.
Induction of programmed cell death by Prangos uloptera, a medicinal plant.
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DOI：
10.4067/s0716-97602009000400013
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发表时间：
2009
影响因子：
6.7
通讯作者：
S. Zahri;S. Razavi;Farshad H Niri;S. Mohammadi
中科院分区：
文献类型：
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作者：
S. Zahri;S. Razavi;Farshad H Niri;S. Mohammadi
抑制细胞生长或诱导细胞死亡是癌症治疗中最有前途的领域。在麦考伊细胞系上评价了Prangos uloptera二氯甲烷提取物对细胞凋亡的诱导作用。这种植物的根、地上部分和果实具有药用价值。采用MTT法和台盼蓝法分别检测提取物对细胞生长的抑制作用和细胞毒性作用。还评价了形态学变化和DNA片段化。处理24小时后，活力试验显示50%抑制浓度和50%细胞毒性浓度分别为0.49和0.3 mg/ml。荧光显微镜分析显示染色质断裂，扫描电子显微镜显示细胞皱缩和细胞质起泡。DNA片段分析证实了这些发现。结果表明，在中等浓度的植物提取物的有效诱导细胞凋亡，但较高浓度的管理表明，细胞死亡的主要方式是坏死。
Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic blebbing. These findings were confirmed by DNA fragmentation analysis. The results demonstrated efficient induction of apoptosis by the plant extract in moderate concentrations, but administration of higher concentrations showed that the primary manner of cell death was necrosis.