Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology.
Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology.
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DOI：
10.1042/bst20160312
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发表时间：
2017-12-15
影响因子：
3.9
通讯作者：
Lomonossoff GP
中科院分区：
文献类型：
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作者：
Meshcheriakova Y;Durrant A;Hesketh EL;Ranson NA;Lomonossoff GP
豇豆花叶病毒（CPMV）颗粒作为纳米颗粒获得了相当大的成功。一种生产病毒空病毒样颗粒（evlp）的系统的开发，增加了基于cpmv的颗粒的可能应用数量。evlp是非传染性的，并且有可能装载异源物质。然而，为了实现这一潜力，必须证明evlp是天然病毒颗粒的准确替代品，这一信息是由evlp的高分辨率冷冻电镜研究提供的。这一演示使得利用天然CPMV颗粒开发的改性颗粒的生产方法能够应用于evlp。此外，低温电镜和诱变研究的结合使粒子的发展成为可渗透的，但仍然可以有效地组装。这些颗粒被证明可以装载钴，这表明它们确实可以用作纳米容器。
Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs. This demonstration has enabled the approaches developed for the production of modified particles developed with natural CPMV particles to be applied to eVLPs. Furthermore, a combination of cryo-EM and mutagenic studies allowed the development of particles which are permeable but which could still assemble efficiently. These particles were shown to be loadable with cobalt, indicating that they can, indeed, be used as nano-containers.