Natural and prosthetic heart valve calcification: morphology and chemical composition characterization.
Natural and prosthetic heart valve calcification: morphology and chemical composition characterization.
复制标题
天然和人工心脏瓣膜钙化：形态和化学成分表征。
作者：
R. F. Weska;C. G. Aimoli;G. M. Nogueira;A. Leirner;M. Maizato;O. Higa;Bronislaw Polakievicz;R. Pitombo;M. Beppu
钙化是心脏瓣膜损伤和随后衰竭的最常见原因。虽然这是一种常见的现象，但对它知之甚少，更少关于从这种类型的钙化中获得的无机相。本文描述了扫描电子显微镜（SEM）/能量色散X射线光谱和Ca K边X射线吸收近边结构（XANES）表征在体内钙化的天然和生物心脏瓣膜（与体外钙化瓣膜相比）中进行。SEM显微照片表明存在相似形态的沉积物，XANES结果表明，在分子水平上，两种类型瓣膜的钙化机制可能相似，导致形成结晶不良的羟基磷灰石沉积物，Ca/P比随时间增加，取决于成熟状态。这些发现可能有助于寻找长期有效的抗钙化治疗。
Calcification is the most common cause of damage and subsequent failure of heart valves. Although it is a common phenomenon, little is known about it, and less about the inorganic phase obtained from this type of calcification. This article describes the scanning electron microscopy (SEM)/energy dispersive X-ray spectroscopy and Ca K-edge X-ray absorption near edge structure (XANES) characterization performed in natural and bioprosthetic heart valves calcified in vivo (in comparison to in vitro-calcified valves). SEM micrographs indicated the presence of deposits of similar morphology, and XANES results indicate, at a molecular level, that the calcification mechanism of both types of valves are probably similar, resulting in formation of poorly crystalline hydroxyapatite deposits, with Ca/P ratios that increase with time, depending on the maturation state. These findings may contribute to the search for long-term efficient anticalcification treatments.