Far-red fluorescent carbon nano-onions as a biocompatible platform for cellular imaging
Far-red fluorescent carbon nano-onions as a biocompatible platform for cellular imaging
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DOI：
10.1039/c7ra09442f
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Giordani, Silvia
中科院分区：
文献类型：
--
作者：
Lettieri, Stefania;Camisasca, Adalberto;Giordani, Silvia
报道了一种新一代的荧光碳纳米洋葱，它在生物介质中具有增强的溶解性和明亮的光致发光。用水溶性硼二吡咯亚甲基染料官能化的纳米洋葱在远红外光谱中发射高量子产率（Phi（F）），并且适合于高分辨率成像。纳米颗粒的特征在于各种不同的分析技术，如热重分析，动态光散射，zeta电位，电子显微镜，拉曼，X-射线光电子和荧光光谱。它们很容易被人乳腺癌细胞（MCF-7）内化，没有任何显著的毒性作用。此外，共聚焦成像研究表明，它们表现出高荧光强度，并以非常低的浓度定位在溶酶体中。我们的研究结果证实了这些功能化碳纳米材料作为高分辨率生物成像的生物相容性平台的优异潜力。
A new generation of fluorescent carbon nano-onions with enhanced solubility in biological media and bright photoluminescence is reported. The nano-onions functionalized with a water soluble boron dipyrromethene dye emit in the far red spectrum with a high quantum yield (Phi(F)) and are suitable for high resolution imaging. The nanoparticles are characterized by a variety of different analytical techniques such as thermogravimetric analysis, dynamic light scattering, zeta potential, electron microscopy, Raman, X-ray photoelectron and fluorescence spectroscopies. They are easily internalized by human breast cancer cells (MCF-7) without any significant toxic effects. Moreover, confocal imaging studies show they exhibit a high fluorescence intensity and are localized in the lysosomes at a very low concentration. Our findings confirm the excellent potentialities of these functionalized carbon nanomaterials as biocompatible platform for high resolution biological imaging.