Evaluation of human sclera after femtosecond laser ablation using two photon and confocal microscopy.
Evaluation of human sclera after femtosecond laser ablation using two photon and confocal microscopy.
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DOI：
10.1117/1.jbo.17.8.081411
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发表时间：
2012-08
影响因子：
3.5
通讯作者：
Hui Sun;R. Kurtz;T. Juhász
中科院分区：
文献类型：
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作者：
Hui Sun;R. Kurtz;T. Juhász
青光眼是世界范围内致盲的第二大原因，通常与眼内压（IOP）升高有关。部分厚度巩膜内通道可以用在1700 nm波长下操作的飞秒激光来创建。这种通道具有增加流出便利性和降低升高的IOP的潜力。分析这些通道的尺寸和位置对于理解它们的影响很重要。我们描述了应用双光子显微镜和共聚焦显微镜的非侵入性成像的飞秒激光创建的部分厚度巩膜通道在人尸体的眼睛。获得巩膜中数百微米深的高分辨率图像，以允许确定这种通道的形状和尺寸。这表明，整合飞秒激光手术、双光子和共焦成像的概念具有在透明和半透明组织中进行图像引导高精度手术的未来潜力。
Glaucoma is the second-leading cause of blindness worldwide and is often associated with elevated intraocular pressure (IOP). Partial thickness intrascleral channels can be created with a femtosecond laser operating at a wavelength of 1700 nm. Such channels have the potential to increase outflow facility and reduce elevated IOP. Analysis of the dimensions and location of these channels is important in understanding their effects. We describe the application of two-photon microscopy and confocal microscopy for noninvasive imaging of the femtosecond laser created partial-thickness scleral channels in human cadaver eyes. High-resolution images, hundreds of microns deep in the sclera, were obtained to allow determination of the shape and dimension of such channels. This demonstrates that concept of integrating femtosecond laser surgery, and two-photon and confocal imaging has the future potential for image-guided high-precision surgery in transparent and translucent tissue.