Examination of the cutaneous absorption of copper after the use of copper-containing ointments.
Examination of the cutaneous absorption of copper after the use of copper-containing ointments.
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DOI：
10.1097/01.mjt.0000127148.83065.e5
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发表时间：
2004-11-01
影响因子：
4.2
通讯作者：
Cobian, Eloy Pulido
中科院分区：
文献类型：
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作者：
Gorter, Robert W;Butorac, Mario;Cobian, Eloy Pulido
由于含铜软膏经常用于人智医学，因此进行了一项 I 期试验来研究铜的皮肤吸收情况。 61 名志愿者被随机分配[A 组：0.4% 氧化铜 (I)，13 名男性和 18 名女性（19-55 岁）； B 组：20% 元素铜，11 名男性和 19 名女性（18-70 岁）]。该药膏使用了 4 周，然后是 4 周的清除阶段。通过原子吸收光谱法测量血清和尿液铜浓度，通过电感耦合等离子体质谱法测量头发铜浓度。对于统计分析，使用相关随机样本的Student t检验；选择 alpha = 0.05 作为标准误差。在A组中，研究期间发现血清和头皮中的铜含量增加，尿液中的铜含量减少。所有使用口服避孕药的绝经前妇女的平均血清浓度均高于正常水平。 B组血清铜浓度显着升高；在尿液中，它下降，而在头皮中，它保持稳定。使用激素避孕的女性志愿者的血清铜水平较高。使用两种不同的软膏治疗不会对皮肤造成毒性刺激，因此可以推断，适当使用含铜浓度高达20%的软膏制剂不会产生毒性风险。
Because copper-containing ointments are frequently used in anthroposophical medicine, a phase I trial to investigate the cutaneous absorption of copper was conducted. Sixty-one volunteers were randomized [group A: 0.4% copper (I) oxide, 13 men and 18 women (19-55 years); group B: 20% elementary copper, 11 men and 19 women (18-70 years)]. The ointment was applied over a 4-week period followed by a 4-week wash-out phase. Serum and urine copper concentrations were measured by atomic absorption spectrometry and hair copper concentration by inductive coupled plasma mass spectrometry. For statistical analysis, the Student t test for related random samples was used; alpha = 0.05 was chosen for the standard error. In group A, an increase of copper in serum and scalp hair and a decrease in urine were found in the study period. The mean serum concentration in all premenopausal women using oral contraceptives was above normal. In group B, the serum copper concentration increased significantly; in urine, it decreased, and in scalp hair, it remained stable. A higher level of serum copper was found in female volunteers using hormonal contraception. Treatment with the 2 different ointments did not cause toxic irritations on the skin, and it can therefore be deduced that the appropriate application of ointment preparations containing copper in concentrations up to 20% do not present a toxic risk.