Facility-wide Eradication of Corynebacterium bovis by using PCR-validated Vaporized Hydrogen Peroxide
Facility-wide Eradication of Corynebacterium bovis by using PCR-validated Vaporized Hydrogen Peroxide
复制标题
DOI：
10.30802/aalas-jaalas-17-000135
复制
发表时间：
2018-09-01
影响因子：
1.7
通讯作者：
Engelman, Robert W.
中科院分区：
文献类型：
--
作者：
Miedel, Emily L.;Ragland, Natalie H.;Engelman, Robert W.
在牛棒杆菌聚合酶链式反应监测的指导下，使用蒸发过氧化氢(VHP)去污建立了设施范围内的牛棒杆菌根除。以前的尝试仅限于扑杀PCR阳性小鼠和净化受影响的房间，但在预防复发方面无效。由于研究目的通常需要贩运到程序核心并使用程序核心，因此对2064个样本进行了12个月的牛链球菌聚合酶链式反应监测，并证明，尽管临床上几乎没有过度角化的小鼠，35%的小鼠的住房和使用空间受到牛链球菌的污染。下腔静脉支架和空气处理单元(AHU)的呼吸道为牛弧菌的存活提供了一个实质性的利基环境，可与初级围栏相媲美，26%的小鼠和22%的呼吸道标本聚合酶链式反应对牛弧菌呈阳性。在“柔性室”中进行设备呼吸道VHP灭菌需要“主动-关闭”设置，将连接到AHU的下腔静脉支架设置为VHP循环，因为来自“静态开放”VHP暴露的呼吸道的12%的样本保持牛链球菌的PCR阳性，而来自主动关闭的VHP暴露的样本的阳性率为0%。29,931-ft2设施的VHP去污工作在2个月内完成。在以前感染牛链球菌的房间对IVC排气室进行了200天的牛链球菌聚合酶链式反应检测，证实所检测的259份样本中没有一份对该微生物的聚合酶链式反应呈阳性。月度监测在2017年6月(9月)期间发现一次复发，确保快速扑杀牛弧菌聚合酶链式反应阳性小鼠，并对设备和房间进行急性VHP净化。在程序和人员领域解决了牛弧菌的分子持久性问题，在11个月和12个月的研究期间，没有小鼠或家畜标本检测牛链球菌PCR阳性。此外，自12个月的研究结束以来，另外452个小鼠、细胞生物学、环境和每月设备监测样本中没有一个牛弧菌检测呈阳性，这记录了到目前为止设施范围内11个月的牛链球菌根除。通过对生物体进行聚合酶链式反应监测，立即扑杀聚合酶链球菌阳性小鼠，以及对受影响地区进行急性VHP净化，可以避免由于牛弧菌引起的研究无效。
Facility-wide Corynebacterium bovis eradication was established using vaporized hydrogen peroxide (VHP) decontamination guided by C. bovis PCR surveillance. Prior attempts limited to culling PCR-positive mice and decontaminating affected rooms were ineffective in preventing recurrence. Because research aims often require trafficking to and use of procedural cores, a 12-mo facility-wide C. bovis PCR surveillance of 2064 specimens was performed and documented that, despite the presence of few clinically hyperkeratotic mice, 35% of the murine housing and use space was contaminated by C. bovis. The airways of IVC racks and air-handling units (AHU) provided a substantive niche for C. bovis survival, comparable to the primary enclosure, with 26% of murine and 22% of airway specimens PCR-positive for C. bovis. Equipment airway VHP sterilization in a 'flex room' required an 'active-closed' setting with the IVC rack connected to the AHU set to the VHP cycle, because 12% of specimens from 'static-open' VHP-exposed airways remained PCR-positive for C. bovis, whereas 0% of specimens from active-closed VHP exposures were positive. VHP decontamination of the 29,931-ft2 facility was completed in 2 mo. C. bovis PCR testing of IVC exhaust plenums for 200 d in previously C. bovis-affected rooms confirmed that none of the 259 specimens tested were PCR-positive for the organism. Monthly surveillance identified a single recurrence during June 2017 (month 9), ensuring rapid culling of C. bovis PCR-positive mice and acute VHP decontamination of equipment and rooms. Molecular persistence of C. bovis was resolved in procedural and personnel areas, and no murine or housing specimens tested C. bovis PCR-positive during study months 11 and 12. Furthermore, since the conclusion of the 12-mo study, none of the 452 additional murine, cell biologic, environmental, and monthly equipment surveillance specimens tested were C. bovis PCR-positive, documenting an 11-mo period of facility-wide C. bovis eradication to date. Study invalidation due to C. bovis can be avoided through PCR surveillance for the organism, immediate culling of PCR-positive mice, and acute VHP decontamination of affected areas.