Bacillus macyae sp nov., an arsenate-respiring bacterium isolated from an Australian gold mine
Bacillus macyae sp nov., an arsenate-respiring bacterium isolated from an Australian gold mine
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DOI：
10.1099/ijs.0.63059-0
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Hoven, RNV
中科院分区：
文献类型：
--
作者：
Santini, JM;Streimann, ICA;Hoven, RNV
从澳大利亚维多利亚本迪戈的一个金矿中分离到一种严格厌氧的砷酸盐呼吸细菌，属于芽孢杆菌属。细胞是革兰氏阳性的活动杆状体，能够以砷酸盐和硝酸盐作为末端电子受体，使用多种底物（包括乙酸盐作为电子供体）进行呼吸。砷酸盐还原为亚砷酸盐是由膜结合的砷酸盐还原酶催化的，该还原酶在宽的pH范围内显示活性。该酶的合成受到调节;当生物体以砷酸盐作为末端电子受体生长时获得最大活性，而当它以硝酸盐生长时没有活性可检测到。基于ingel活性染色，催化亚基的质量被确定为约87 kDa。基于16 S rRNA基因序列分析，最接近的系统发育关系是砷硒芽孢杆菌，但DNA-DNA杂交实验清楚地表明菌株JMM-4（T）代表一个新的芽孢杆菌属物种，建议将其命名为Bacillus macyae sp. nov.。模式菌株为JMM-4（T）（= DSM 16346（T）=JCM 12340（T））。
A strictly anaerobic arsenate-respiring bacterium isolated from a gold mine in Bendigo, Victoria, Australia, belonging to the genus Bacillus is described. Cells are Gram-positive, motile rods capable of respiring with arsenate and nitrate as terminal electron acceptors using a variety of substrates, including acetate as the electron donor. Reduction of arsenate to arsenite is catalysed by a membrane-bound arsenate reductase that displays activity over a broad pH range. Synthesis of the enzyme is regulated; maximal activity is obtained when the organism is grown with arsenate as the terminal electron acceptor and no activity is detectable when it is grown with nitrate. Mass of the catalytic subunit was determined to be approximately 87 kDa based on ingel activity stains. The closest phylogenetic relative, based on 16S rRNA gene sequence analysis, is Bacillus arseniciselenatis, but DNA-DNA hybridization experiments clearly show that strain JMM-4(T) represents a novel Bacillus species, for which the name Bacillus macyae sp. nov. is proposed. The type strain is JMM-4(T) ( = DSM 16346(T)=JCM 12340(T)).