Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy.
Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy.
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DOI：
10.1364/ol.35.004018
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发表时间：
2010-12-01
期刊：
影响因子：
3.6
通讯作者：
Jiao S
中科院分区：
文献类型：
--
作者：
Zhang X;Jiang M;Fawzi AA;Li X;Shung KK;Puliafito CA;Zhang HF;Jiao S
We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.
