Porous 3D Printed Scaffolds For Guided Bone Regeneration In a Rat Calvarial Defect Model
Porous 3D Printed Scaffolds For Guided Bone Regeneration In a Rat Calvarial Defect Model
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DOI：
10.1016/j.apmt.2020.100706
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发表时间：
2020-09-01
影响因子：
8.3
通讯作者：
Tran, Phong A.
中科院分区：
文献类型：
--
作者：
Dang, Hoang Phuc;Vaquette, Cedryck;Tran, Phong A.
在以前的研究中已经探索了3D打印支架用于骨组织再生的应用。在这项研究中，我们将3D打印与致孔剂浸出相结合，开发具有双尺度孔隙率的支架，并研究其在大鼠临界尺寸颅骨缺损模型中引导骨再生的能力。支架由掺杂有平均尺寸为22 μ m的致孔剂微粒的医用级聚己内酯（mPCL）增材制造，随后将其沥滤以产生微尺度孔隙。形态学分析揭示了约60%的平均孔径为700 μ m的互连宏观孔隙率和孔隙率接近40%的平均孔径为20-70 μ m的支柱内微观孔隙。微尺度孔隙率导致支架的表面积增加3倍，带负电荷的表面基团富集2倍，这确实导致蛋白质吸附显著增加和体外更快的水解驱动降解。体外血液凝固试验表明，从双尺度多孔支架上形成的凝块中释放的TGF-β 1增加。在大鼠颅骨缺损中，在宏观和微观孔中均发现骨形成，并且与磷酸钙涂层mPCL支架相比处于相似水平。皇冠版权所有（c）2020由爱思唯尔有限公司出版。保留所有权利。
The application of 3D printed scaffolds f or bone tissue regeneration has been explored in previous studies. In this study, we combined 3D printing with porogen leaching to develop scaffolds with dual-scale porosity and investigated their capability in guided bone regeneration in a rat critical size calvarial defect model. The scaffolds were additively manufactured from medical grade polycaprolactone (mPCL) doped with porogen microparticles having an average size of 22 mu m, which were subsequently leached to create microscale porosity. Morphological analysis revealed an interconnected macroscale porosity of about 60% with an average pore size of 700 mu m and intra-strut microscale pores with a porosity of nearly 40% and average pore size of 20-70 mu m. The microscale porosity resulted in a 3-fold increase in the scaffolds' surface area, a 2-fold enrichment in negatively charged surface groups, which did lead to significantly increased protein adsorption and faster hydrolysis-driven degradation in vitro . An in vitro blood clotting assay demonstrated an increased TGF-beta 1 release from the clots formed on the dual-scale porous scaffolds. In a rat calvarial defect, bone formation was found in both the macroand microscale pores and was at a similar level when compared to calcium phosphate coated mPCL scaffolds. Crown Copyright (c) 2020 Published by Elsevier Ltd. All rights reserved.