LIPID EXTRACTION OF TISSUES WITH A LOW-TOXICITY SOLVENT
LIPID EXTRACTION OF TISSUES WITH A LOW-TOXICITY SOLVENT
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DOI：
10.1016/0003-2697(78)90046-5
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
RADIN, NS
中科院分区：
文献类型：
--
作者：
HARA, A;RADIN, NS
从组织[大鼠或小鼠脑]中提取脂质的改进方法包括使用己烷：异丙醇，然后用硫酸钠水溶液洗涤提取物以除去非脂质污染物。这种方法比通常使用的氯仿：甲醇有许多优点。溶剂的毒性稍低，避免了蛋白脂质蛋白质污染对处理的干扰，在洗涤步骤中两相迅速分离，溶剂密度足够低以允许匀浆的离心作为过滤的替代方案，溶剂更便宜，并且洗涤的提取物可以应用于色谱柱，在远UV区域连续监测洗脱。新的提取方法对神经节苷脂的提取效率低。
An improved method for extracting the lipids from tissues [rat or mouse brain] consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants. This method has a number of advantages over the common usage of chloroform:methanol. The solvents are somewhat less toxic, interference in processing by proteolipid protein contamination is avoided, the 2 phases separate rapidly during the washing step, the solvent density is low enough to permit centrifugation of the homogenate as an alternative to filtration, the solvents are cheaper, and the washed extract can be applied to a chromatographic column with continuous monitoring of the elution in the far UV region. The new extraction method is inefficient for the extraction of gangliosides.