Immunohistochemical detection of PrP in the medulla oblongata of sheep: the spectrum of staining in normal and scrapie-affected sheep
Immunohistochemical detection of PrP in the medulla oblongata of sheep: the spectrum of staining in normal and scrapie-affected sheep
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DOI：
10.1136/vr.148.1.7
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发表时间：
2001-01-06
影响因子：
2.2
通讯作者：
March, SA
中科院分区：
文献类型：
--
作者：
Ryder, SJ;Spencer, YI;March, SA
用免疫组织化学方法对绵羊脑延髓组织中朊蛋白（PrP）进行了检测。的沉积物的形态和神经解剖分布的基础上，不同的疾病相关的模式的PrP沉积被确定在羊瘙痒症影响的羊，这表明至少有四个不同的表型的羊瘙痒症。此外，明确定义的模式的PrP沉积，很容易区分与疾病相关的PrP存款，确定在一些正常的羊从羊瘙痒症免费羊群。在5只羊，被认为是临床前受瘙痒症，PrP沉积的疾病特异性类型，但分布有限的确定，证明了敏感性的技术诊断瘙痒症。这些早期PrP沉积物的神经解剖学分布表明，羊瘙痒病因子进入大脑的途径是通过支配胃肠道的迷走神经中的副交感运动神经元。
Sections of the medulla oblongata from the brains of sheep were examined for prion protein (PrP) by immunohistochemistry. On the basis of the morphology and neuroanatomical distribution of the deposits, distinct disease-associated patterns of PrP deposition were identified in scrapie-affected sheep, suggesting at least four distinct phenotypes of scrapie. In addition, clearly defined patterns of PrP deposition, readily distinguished from the disease-associated PrP deposits, were identified in some normal sheep from scrapie-free flocks. In five sheep, believed to be preclinically affected by scrapie, PrP deposition of a disease-specific type but of restricted distribution was identified, demonstrating the sensitivity of the technique for the diagnosis of scrapie. The neuroanatomical distribution of these early PrP deposits suggest that the route of entry of the scrapie agent into the brain is via parasympathetic motor neurons in the vagus nerve which innervate the gastrointestinal tract.