Differential pattern of indigenous microbiome responses to probiotic Bifidobacterium lactis V9 consumption across subjects
Differential pattern of indigenous microbiome responses to probiotic Bifidobacterium lactis V9 consumption across subjects
复制标题
不同受试者对益生菌乳双歧杆菌 V9 消耗的本土微生物组反应的差异模式
DOI：
10.1016/j.foodres.2020.109496
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发表时间：
2020-10-01
影响因子：
8.1
通讯作者：
Zhang, Heping
中科院分区：
文献类型：
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作者：
Ma, Chenchen;Huo, Dongxue;Zhang, Heping
为了进一步探索益生菌的健康促进作用，应考虑各种因素，包括与宿主和环境相关的因素。然而，重要的是要考虑持续性是益生菌在肠道中功能的基本但关键的因素。迄今为止，很少有研究调查影响益生菌持久性的因素。为了解决这些挑战，我们设计了一个包括49名受试者的队列实验，并使用益生菌乳酸双歧杆菌V9，基于高通量扩增子测序鉴定与益生菌持久性相关的肠道微生物群。根据粪便中乳酸双歧杆菌V9活菌的检出量将所有受试者分为持续者、暂时者和非持续者3组。因此，坚持组的肠道菌群波动显著且持续，而非坚持组观察到的肠道菌群波动有限。在属水平上，持续菌组有7个属发生了显著变化，而非持续菌组只有厌氧菌属发生了显著变化。在基线时，我们观察到在坚持组和非坚持组之间高度不同的微生物α多样性和分类特征。共有12个属与益生菌持久性相关，其中双歧杆菌和其他8个属与益生菌持久性负相关，厌氧菌、副revotella和丹毒杆菌与益生菌持久性正相关。基于这些潜在的生物标志物，导出了“抗植入指数”（AEI），用于分类和预测WA和验证队列中益生菌的持久性，准确度很高。然而，我们也观察到AEI在其他益生菌消耗实验中不起作用，这表明AEI是菌株特异性的。
Various factors, including those associated with the host and environment, should be considered to further explore the health-promoting effects of probiotics. However, it is important to consider persistence as a basic but crucial factor in the function of probiotics in the gut. To date, few studies have investigated the factors that influence probiotic persistence. To address these challenges, we designed a cohort experiment that included 49 subjects and used the probiotic Bifidobacterium lactis V9 to identify intestinal microbiota related to probiotic persistence based on high-throughput amplicon sequencing. All of the subjects were divided into three groups (Persisters, Temporary and Non-Persisters) according to the detected amount of viable Bifidobacterium lactis V9 in their faeces. Accordingly, the intestinal microbiota fluctuations in the Persisters group were significant and persistent, whereas those observed in the Non-Persisters group were limited. At the genus level, up to seven genera changed significantly in Persisters group, whereas only the genus Anaerobacterium changed significantly in Non-Persisters group throughout the experiment. At baseline, we observed highly distinct microbial alpha diversity and taxonomic features between the Persisters and Non-Persisters groups. A total of 12 genera were associated with probiotic persistence, with Bifidobacterium and eight other genera negatively associated with probiotic persistence and Anaerobacterium, Paraprevotella and Erysipelatoclostridium positively associated with probiotic persistence. Based on these potential biomarkers, an "Anti-Engraftment Index" (AEI) was derived to classify and predict probiotic persistence in WA and validation cohorts with high accuracy. However, we also observed that the AEI did not work in other probiotic consumption experiments, indicating that the AEI was strain-specific.