Nucleic Acid Immunizations
Nucleic Acid Immunizations
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DOI：
10.1002/0471142735.im0214s27
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发表时间：
1998-09
影响因子：
--
通讯作者：
H. Robinson;T. Pertmer
中科院分区：
文献类型：
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作者：
H. Robinson;T. Pertmer
该单元提供了两种广泛使用的DNA免疫方法的协议：盐水注射和DNA的基因枪交付。盐水注射将DNA输送到细胞外空间;基因枪输送将DNA直接轰击到细胞内。支持方案提供了用于制备DNA包被的金珠、创建含有这些珠的盒以及优化基因枪参数的方法。有关质粒载体，DNA制备和剂量，免疫方案的问题也进行了讨论。表达库免疫，遗传和常规佐剂，替代性加强，新生儿免疫，以及粘膜递送DNA的方法都包括在评论中。
This unit provides protocols for two broadly used methods of DNA immunization: saline injections and gene gun deliveries of DNA. Saline injections deliver DNA into extracellular spaces; gene gun deliveries bombard DNA directly into cells. Support protocols present methods for preparation of DNA‐coated gold beads, creation of cartridges containing these beads, and optimization of gene gun parameters. Issues relating to plasmid vectors, DNA preparation and doses, and immunization regimens are also discussed. Expression library immunizations, genetic and conventional adjuvants, alternative boosts, neonatal immunizations, and approaches to mucosal delivery of DNA are covered in the commentary.