Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages
Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages
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DOI：
10.1182/blood-2004-03-1140
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发表时间：
2004-12-15
期刊：
影响因子：
20.3
通讯作者：
Brown, GD
中科院分区：
文献类型：
--
作者：
Herre, J;Marshall, ASJ;Brown, GD
病原体的吞噬作用是宿主防御中的关键事件，不仅对入侵的微生物有清除作用，而且对随后的免疫反应也有重要作用。我们研究了Dectin-1，一种β-葡聚糖的促炎性非调理受体，并表明它介导了含P-葡聚糖的配体的内化，包括酵母颗粒。虽然需要酪氨酸磷酸化和胞浆免疫受体酪氨酸激活基序(ITAM)样基序，但Dectin-1介导的摄取不同于以往报道的任何吞噬细胞受体，并且不依赖于巨噬细胞中的Syk-Kinase。此外，该受体在细胞内的转运受到P-葡聚糖配体性质的影响，这对这些免疫调节碳水化合物的生物活性具有重要意义。(C)2004年，由美国血液病学会提供。
The phagocytosis of pathogens is a critical event in host defense, not only for clearance of the invading microorganism, but also for the subsequent immune response. We have examined Dectin-1, a proinflammatory nonopsonic receptor for beta-glucans, and show that it mediates the internalization of P-glucan-bearing ligands, including yeast particles. Although requiring tyrosine phosphorylation and the cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM)-like motif, uptake mediated by Dectin-1 was different from any previously reported phagocytic receptor and was not dependent on Syk-kinase in macrophages. Furthermore, intracellular trafficking of this receptor was influenced by the nature of the P-glucan ligand, which has significance for the biologic activity of these immunomodulatory carbohydrates. (C) 2004 by The American Society of Hematology.