Phenotypic and Physiological Changes in Acinetobacter sp. Strain DR1 with Exogenous Plasmid
Phenotypic and Physiological Changes in Acinetobacter sp. Strain DR1 with Exogenous Plasmid
复制标题
不动杆菌的表型和生理变化。
作者：
Jungsoon Park;Woojun Park
不动杆菌属已被认为可以从环境中吸收外源DNA。在本研究中，我们对一株新型柴油降解不动杆菌进行了自然转化。菌株，命名为菌株DR1，使用广泛宿主范围的质粒pRK415。许多因素，包括温度、DNA含量和曝气量，已被证明对有效的自然转化至关重要。有趣的是，不动杆菌属。菌株DR1(PRK415)在表型和生理上都与野生型菌株不同，包括运动性、生物膜形成能力和对氧化胁迫的反应：转化细胞对过氧化氢和过氧化异丙苯更加敏感，其运动性和生物膜形成活性也减弱。我们的数据表明，在用质粒进行遗传操作时应该谨慎，因为寄主的表型和生理变化可能会随着质粒的摄取而发生。
The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA, and aeration have proven critically important for efficient natural transformation. Interestingly, the Acinetobacter sp. strain DR1 (pRK415) differed both phenotypically and physiologically from the wild-type strain in several regards, including motility, biofilm formation ability, and responses to oxidative stress: the transformed cells were rendered more sensitive to hydrogen peroxide and cumene hydroperoxide, and their motilities and biofilm formation activity were also attenuated. Our data demonstrated that caution should be exercised when conducting genetic manipulation with plasmids, due to the possibility that phenotypic and physiological changes in the host might occur along with the uptake of plasmids.
影响因子：
16
作者：
Kohanski MA;DePristo MA;Collins JJ
通讯作者：
Collins JJ