Significance of medial preoptic area among the subcortical and cortical areas that are related to pain regulation in the rats with stress-induced hyperalgesia
Significance of medial preoptic area among the subcortical and cortical areas that are related to pain regulation in the rats with stress-induced hyperalgesia
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DOI：
10.1016/j.brainres.2020.146758
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发表时间：
2020-03
期刊：
影响因子：
2.9
通讯作者：
H. Imbe;A. Kimura
中科院分区：
文献类型：
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作者：
H. Imbe;A. Kimura
心理物理应激经常增加对疼痛的敏感性和反应，这被称为应激诱导的痛觉过敏（SIH）。然而，其机制仍然未知。视前内侧核（MPO）、下丘脑背内侧核（DMH）、杏仁核基底外侧核（BLA）和中央核（CeA）等皮质下区以及岛叶（IC）和前扣带皮质（ACC）等皮质区通过下行痛觉调制系统在痛觉控制中起重要作用。在本研究中，我们研究了磷酸化cAMP反应元件结合蛋白（pCREB）的表达和组蛋白H3的乙酰化在这些皮层下和皮层区域反复束缚应激后，揭示在皮层下和皮层区域的变化，影响下行疼痛调节系统的功能在SIH大鼠。重复束缚应激3周可引起大鼠后爪机械阈值降低，MPO中pCREB表达增加，MPO、BLA和IC中组蛋白H3乙酰化水平升高。MPO是唯一的区域，显示在这些检查区域中的pCREB的表达和组蛋白H3的乙酰化的增加后，重复束缚应激。此外，MPO中pCREB-IR或乙酰化组蛋白H3-IR细胞的数量与机械阈值呈负相关。总之，我们的数据代表的重要性，MPO皮质下和皮质区控制下行疼痛调制系统下SIH的条件。
Psychophysical stresses frequently increase sensitivity and response to pain, which is termed stress-induced hyperalgesia (SIH). However, the mechanism remains unknown. The subcortical areas such as medial preoptic area (MPO), dorsomedial nucleus of the hypothalamus (DMH), basolateral (BLA) and central nuclei of the amygdala (CeA), and the cortical areas such as insular (IC) and anterior cingulate cortices (ACC) play an important role in pain control via the descending pain modulatory system. In the present study we examined the expression of phosphorylated -cAMP-response element binding protein (pCREB) and the acetylation of histone H3 in these subcortical and cortical areas after repeated restraint stress to reveal changes in the subcortical and cortical areas that affect the function of descending pain modulatory system in the rats with SIH. The repeated restraint stress for 3 weeks induced a decrease in mechanical threshold in the rat hindpaw, an increase in the expression of pCREB in the MPO and an increase in the acetylation of histone H3 in the MPO, BLA and IC. The MPO was the only area that showed an increase in both the expression of pCREB and the acetylation of histone H3 among these examined areas after the repeated restraint stress. Furthermore, the number of pCREB-IR or acetylated histone H3-IR cells in the MPO was negatively correlated with the mechanical threshold. Together, our data represent the importance of the MPO among the subcortical and cortical areas that control descending pain modulatory system under the condition of SIH.