Epithelial effects on limb chondrogenesis involve extracellular matrix and cell shape.
Epithelial effects on limb chondrogenesis involve extracellular matrix and cell shape.
复制标题
上皮对肢体软骨形成的影响涉及细胞外基质和细胞形状。
DOI：
10.1016/0012-1606(86)90113-2
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Solursh,M
中科院分区：
文献类型：
--
作者：
Zanetti,NC;Solursh,M
肢芽间充质细胞的胶原凝胶培养物通常允许软骨形成，但当它们被肢外胚层预处理时，对软骨形成具有抑制作用。这种抑制对软骨分化是特异性的，因为成肌细胞分化不受影响并且变平，成纤维细胞在条件凝胶上更多。外胚层条件凝胶的抗软骨形成作用不被升高细胞内环AMP水平和在其他条件下促进软骨形成的试剂阻断。相比之下，当用细胞松弛素D处理培养物时，外胚层的抑制作用减轻，细胞松弛素D是一种导致细胞保持球形的细胞分裂素破坏剂。这些结果表明，外胚层条件胶原蛋白凝胶抑制软骨形成通过对细胞形状的影响。
Collagen gel cultures of limb bud mesenchymal cells are normally permissive for chondrogenesis but become inhibitory for chondrogenesis when they are preconditioned by limb ectoderm. This inhibition is specific for cartilage differentiation, inasmuch as myoblast differentiation is unaffected and flattened, fibroblastic cells are more numerous on conditioned gels. The antichondrogenic effect of ectoderm-conditioned gels is not blocked by agents that elevate intracellular cyclic AMP levels and that promote chondrogenesis under other conditions. In contrast, the inhibitory effect of the ectoderm is alleviated when cultures are treated with cytochalasin D, a cytoskeleton-disrupting agent that causes the cells to remain spherical. These results suggest that ectoderm-conditioned collagen gels inhibit chondrogenesis through an effect on cell shape.