Aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance.
Aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance.
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埃及伊蚊铁蛋白重链同源物：铁或血液的喂养会影响信息水平、长度和亚基丰度。
DOI：
10.1093/jis/2.1.7
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Law,JohnH
中科院分区：
文献类型：
--
作者：
Dunkov,BorisC;Georgieva,Teodora;Yoshiga,Toyoshi;Hall,Martin;Law,JohnH
埃及伊蚊分泌的铁蛋白有几个亚基，它们是至少两个基因的产物，一个编码脊椎动物重链同源物（HCH），另一个编码轻链同源物（LCH）。在这里，我们报告的发展和器官的具体模式表达的铁蛋白六氯环己烷的消息和两个亚基类型在控制糖喂养的蚊子，在那些暴露于高水平的膳食铁，和后bloodfeeding.When北方印迹探测与六氯环己烷cDNA，观察到两条带，代表至少两个不同大小的消息，结果从两个不同的多聚腺苷酸化位点的选择。无论是在富含铁的培养基中饲养蚊子幼虫，还是吸血成年雌蚊，都导致六氯环己烷信息水平显著增加，特别是较短的信息。研究了在蚊的整个生命周期内和吸血后雌蚊不同器官中信息量和长度以及铁蛋白亚基量的变化。吸血昆虫的中肠是铁蛋白信息合成增加的主要场所。摄食后中肠、脂肪体和血淋巴中铁蛋白水平也增加。铁蛋白信息和亚基在卵巢中合成，铁蛋白在卵子中发现。这些意见进行了讨论，在铁蛋白合成的翻译和转录控制方面，并进行了比较，以类似的事件在调节ofDrosophila melanogasterferritin。
Secreted ferritin in the mosquito,Aedes aegypti, has several subunits that are the products of at least two genes, one encoding a homologue of the vertebrate heavy chain (HCH) and the other the light chain homologue (LCH). Here we report the developmental and organ specific pattern of expression of the ferritin HCH messages and of both subunit types in control sugar-fed mosquitoes, in those exposed to high levels of dietary iron, and after blood feeding.When Northern blots were probed with a HCH cDNA, two bands were observed, representing at least two messages of different sizes that result from the choice of two different polyadenylation sites. Either raising mosquito larvae in an iron-enriched medium, or blood feeding adult female mosquitoes resulted in a marked increase in the HCH message level, particularly of the shorter message. Changes in the amount and length of messages and amount of ferritin subunits were studied over the life span of the mosquito and in different organs of female mosquitoes after blood feeding. The midgut of blood-fed insects is the main site of increased ferritin message synthesis. Ferritin protein levels also increase in midgut, fat body and hemolymph after blood feeding. Ferritin messages and subunits are synthesized in the ovaries and ferritin is found in the eggs. These observations are discussed in terms of translational and transcriptional control of ferritin synthesis and are compared to similar events in the regulation ofDrosophila melanogasterferritin.