Identification of the Main Toxins Isolated from Fusarium oxysporum f. sp pisi Race 2 and Their Relation with Isolates' Pathogenicity
Identification of the Main Toxins Isolated from Fusarium oxysporum f. sp pisi Race 2 and Their Relation with Isolates' Pathogenicity
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DOI：
10.1021/jf405530g
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发表时间：
2014-03-26
影响因子：
6.1
通讯作者：
Cirnmino, Alessio
中科院分区：
文献类型：
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作者：
Bani, Moustafa;Rispail, Nicolas;Cirnmino, Alessio
尖孢镰刀菌Fusarium oxporum f.sp.豌豆纹枯病(PISI，FOP)是引起豌豆严重维管束萎病的主要病原菌。植物对产生枯萎症状的1、5和6号小种的抗性是由一个显性基因决定的，而对2号小种的抗性是最近被证明是数量的。在据报道在感染过程中发挥作用的毒力因素中，毒素产生是研究得最好的因素之一。因此，对5个小种2菌株的体外毒素产生及其与菌株致病性的关系进行了研究。所有菌株都能产生不同量的褐腐酸和9，10-脱氢褐腐酸。这两种毒素的含量已被定量，并与田间豌豆分离物的致病性和侵袭性相关。结果表明，毒素的产生是FOP小种2致病力的重要决定因素。
Fusarium oxysporum f. sp. pisi (Fop) is a pathogen of field pea inducing severe vascular wilt worldwide. Plant resistance to races 1, 5, and 6, producing wilt symptoms, is conferred by a single dominant gene, while resistance to race 2, which gives near-wilt symptoms, have been recently showed to be quantitative. Among the virulence factors reported to play a role in the infection process, toxin production is one of the best studied. Thus, five race 2 isolates have been investigated for toxin production in vitro and their relation to isolates' pathogenicity. All the isolates produced different amounts of fusaric and 9,10-dehydrofusaric acids. The content of the two toxins has been quantitated and correlated with the pathogenicity and aggressiveness of isolates on field pea. Results suggested that toxin production is an important determinant of Fop race 2 pathogenicity.