Renaissance for Phage-Based Bacterial Control
Renaissance for Phage-Based Bacterial Control
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DOI：
10.1021/acs.est.1c06232
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发表时间：
2022-04-19
影响因子：
11.4
通讯作者：
Alvarez, Pedro J. J.
中科院分区：
文献类型：
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作者：
Schwarz, Cory;Mathieu, Jacques;Alvarez, Pedro J. J.
噬菌体（Bacteriophages，简称BPHs）是一种未充分利用的生物资源，在病原体控制和微生物组编辑方面具有巨大潜力。噬菌体的研究和商业化在生物医学和农业产业中迅速发展，但在其他领域的应用却很有限。尽管如此，DNA测序、生物信息学、微生物生态学和合成生物学的融合进展现在正准备扩大噬菌体的应用范围，使其超越病原体控制，转向操纵微生物群落以实现明确的功能改进。测序与网络分析相结合的增强功能使得现在可以识别和破坏微生物协会，以引起社区结构或功能的理想转变，间接调节物种丰度，并针对枢纽或关键物种进行广泛的功能转变。测序和生物信息学的进步也促进了温和测序在安全基因递送应用中的应用。最后，合成生物学的整合将创造新的噬菌体底盘和模块化遗传组件。尽管一些基础、监管和商业化的障碍仍然阻碍着噬菌体的广泛使用，但阻碍该领域的许多重大挑战现在都有了可行的解决方案。因此，基于噬菌体（无化学品）的精确生物控制和微生物组编辑的新曙光即将到来，以增强，抑制或调节微生物活性，这对公共卫生，食品安全以及更可持续的能源生产和水再利用至关重要。
Bacteriophages (phages) are an underutilized biological resource with vastpotential for pathogen control and microbiome editing. Phage research andcommercialization have increased rapidly in biomedical and agricultural industries, butadoption has been limited elsewhere. Nevertheless, converging advances in DNAsequencing, bioinformatics, microbial ecology, and synthetic biology are now poised tobroaden phage applications beyond pathogen control toward the manipulation ofmicrobial communities for defined functional improvements. Enhancements insequencing combined with network analysis make it now feasible to identify and disruptmicrobial associations to elicit desirable shifts in community structure or function,indirectly modulate species abundance, and target hub or keystone species to achievebroad functional shifts. Sequencing and bioinformatic advancements are also facilitatingthe use of temperate phages for safe gene delivery applications. Finally, integration ofsynthetic biology stands to create novel phage chassis and modular genetic components.While some fundamental, regulatory, and commercialization barriers to widespread phageuse remain, many major challenges that have impeded thefield now have workable solutions. Thus, a new dawn for phage-based(chemical-free) precise biocontrol and microbiome editing is on the horizon to enhance, suppress, or modulate microbial activitiesimportant for public health, food security, and more sustainable energy production and water reuse.