Electron microscopic observation of photoreceptor cells in directly inserted anesthetized Drosophila into a high-pressure freezing unit
Electron microscopic observation of photoreceptor cells in directly inserted anesthetized Drosophila into a high-pressure freezing unit
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直接插入高压冷冻装置的麻醉果蝇感光细胞的电子显微镜观察
DOI：
10.1002/jemt.23166
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Terada N
中科院分区：
文献类型：
--
作者：
Terada H;Saitoh Y;Kametani K;Sakaguchi M;Sakamoto T;Kamijo A;Terada N
已知高压冷冻（HPF）技术与其他冷冻技术相比，可以冷冻固定含水材料，在深层几乎没有冰晶形成。本研究将麻醉的活体果蝇直接置于HPF装置的载体上并暴露于光下进行HPF。将冷冻果蝇冰冻后，用透射电镜观察复眼。由感光细胞组成的小眼超微结构保存完好。光感受器细胞内的细胞器的位置进行了观察。在光暴露果蝇小眼的某些感光细胞中，与非光暴露果蝇相比，细胞质小颗粒位于更靠近杆粒基部的位置。因此，HPF与直接插入livingDrosophila在光照下进入HPF机使我们能够检查变化的感光细胞的功能结构发生在几秒钟内。
The high‐pressure freezing (HPF) technique is known to cryofix water‐containing materials with little ice‐crystal formation in deep depths compared with other freezing techniques. In this study, HPF for anesthetized livingDrosophilawas performed by placing them directly on the carrier of the HPF unit and exposing them to light. FrozenDrosophilawere freeze substituted, and their compound eyes were examined by transmission electron microscopy. The ultrastructures of ommatidia composed of photoreceptor cells were well preserved. The location of the cytoplasmic organelles inside the photoreceptor cells was observed. In some photoreceptor cells in ommatidia of the light‐exposedDrosphila, the cytoplasmic small granules were localized nearer the base of rhabdomeres, compared with those of the nonlight‐exposedDrosophila. Thus, HPF with the direct insertion of livingDrosophilaunder light exposure into the HPF machine enabled us to examine changes to functional structures of photoreceptor cells that occur within seconds.