High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia
High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia
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DOI：
10.1529/biophysj.107.116491
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发表时间：
2008-01-15
影响因子：
3.4
通讯作者：
Dufrene, Yves F.
中科院分区：
文献类型：
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作者：
Dague, Etienne;Alsteens, David;Dufrene, Yves F.
我们使用实时原子力显微镜和温度控制阶段(37℃)来探索单个烟曲霉分生孢子在萌发过程中的结构和物理化学动力学。休眠孢子的纳米级地形图像显示，存在一层由疏水素制成的小棒状结构，这与早先的电子显微镜观察结果一致。在3h的萌发期内，观察到小棒层逐渐破裂，显示出亲水性的内细胞壁结构。使用疏水尖端的粘附力作图，这些超微结构的变化被证明与细胞表面疏水性的主要差异有关。也就是说，由于疏水素的存在，小棒表面是均匀疏水的，而萌发时出现的细胞壁材料是纯粹的亲水性。这项研究展示了实时原子力显微镜成像和力光谱在跟踪细胞表面动力学方面的潜力。
We used real-time atomic force microscopy with a temperature-controlled stage (37 degrees C) to probe the structural and physicochemical dynamics of single Aspergillus fumigatus conidia during germination. Nanoscale topographic images of dormant spores revealed the presence of a layer of rodlets made of hydrophobins, in agreement with earlier electron microscopy observations. Within the 3-h germination period, progressive disruption of the rodlet layer was observed, revealing hydrophilic inner cell wall structures. Using adhesion force mapping with hydrophobic tips, these ultrastructural changes were shown to correlate with major differences in cell surface hydrophobicity. That is, the rodlet surface was uniformly hydrophobic due to the presence of hydrophobins, whereas the cell wall material appearing upon germination was purely hydrophilic. This study illustrates the potential of real-time atomic force microscopy imaging and force spectroscopy for tracking cell-surface dynamics.