Analyses of the floral organ morphogenesis and the differentially expressed genes of an apetalous flower mutant in Brassica napus
Analyses of the floral organ morphogenesis and the differentially expressed genes of an apetalous flower mutant in Brassica napus
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DOI：
10.1007/s00299-007-0426-4
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发表时间：
2007-09
影响因子：
6.2
通讯作者：
Y. Zhou;H. Y. Wang;L. Zhou;M. P. Wang;H. Li;M. Wang;Y. Zhao
中科院分区：
文献类型：
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作者：
Y. Zhou;H. Y. Wang;L. Zhou;M. P. Wang;H. Li;M. Wang;Y. Zhao
对甘蓝型油菜无花瓣花突变体Apet 33 -10花器官形态发生进行了研究，结果表明：在花发育过程中，除花瓣原基未出现外，其余花器官形态发生正常。利用抑制性消减杂交（SSH）和RT-PCR技术，在Apet 33 -10的早期花芽（小于200 μm）中发现18个基因在花器官分化期表达下调。这些基因与花瓣的特性、钙铁信号转导、mRNA加工、蛋白质合成与降解、细胞骨架的构建、氢运输、核酸结合、生物碱生物合成以及未知功能有关。通过RT-PCR方法获得APETALA 3（AP 3）基因的三个全编码区cDNA，分别为BnAP 3 -2、BnAP 3 - 3和BnAP 3 - 4。实时荧光定量PCR分析结果表明，野生型Pet 3 - 3 -10早期花芽中BnAP 3 -2、BnAP 3 - 3和BnAP 3 - 4的表达比例为3.67：3.68：1。Apet 3 - 3 -10早期花芽中BnAP 3 -2、BnAP 3 - 3和BnAP 3 - 4的表达量分别比野生型低36.6%、28.3%和66.8%，无花瓣突变体中AP 3基因的总表达量为野生型的45.0%。各AP 3基因在无花瓣和野生型品系雄蕊中的表达量差异不显著。这表明，AP 3基因在Apet 33 -10花发育早期的低丰度表达可能足以促进雄蕊原基的发生，但不足以促进花瓣原基的发生。
The floral organ morphogenesis of the apetalous flower mutant Apet33-10 inBrassica napuswas investigated and the result showed that all the floral organ morphogenesis was normal except that petal primordium was not observed during flower development. Eighteen genes were found to be down regulated in early floral buds (less than 200 μm in length) of Apet33-10 at the stage of floral organ initiation by means of suppressive subtraction hybridization (SSH) and RT-PCR. These genes were involved in petal identity, calcium iron signal transduction, mRNA processing, protein synthesis and degradation, construction of cytoskeleton, hydrogen transportation, nucleic acid binding, alkaloid biosynthesis and unknown function. Three overall coding region cDNAs ofAPETALA3 (AP3) gene,BnAP3-2, BnAP3-3andBnAP3-4were obtained by RT-PCR, respectively. Real-time quantitative PCR analysis showed that the expression ratio amongBnAP3-2,BnAP3-3andBnAP3-4was 3.67:3.68:1 in early floral buds of wild type Pet33-10. The expression level ofBnAP3-2,BnAP3-3andBnAP3-4in early floral buds of Apet33-10 was down-regulated to 36.6, 28.3 and 66.8% with the comparison of that of wild type, respectively, and the overall expression level ofAP3genes in apetalous mutant amounted to 45.0% of that in wild type. The difference in the expression level of eachAP3gene in stamen between apetalous and wild type lines was not significant. It is suggested that lower abundant expression ofAP3genes during the early flower development might be enough for stamen primordium initiation, but not enough for petal primordium initiation in the apetalous line Apet33-10.