Alleviation effects of natural volatile organic compounds from Pinus densiflora and Chamaecyparis obtusa on systemic and pulmonary inflammation
Alleviation effects of natural volatile organic compounds from Pinus densiflora and Chamaecyparis obtusa on systemic and pulmonary inflammation
复制标题
DOI：
10.3892/br.2018.1147
复制
发表时间：
2018-11-01
影响因子：
2.3
通讯作者：
Jeung, Eui-Bae
中科院分区：
文献类型：
--
作者：
Ahn, Changhwan;Lee, Jae-Hwan;Jeung, Eui-Bae
Chamaaparis obtusa（C. obtusa）和赤松（Pinusdensiflora）在亚洲民间医学中传统上被用作抗生素、抗伤害剂和抗炎剂。近年来的研究表明，C. obtusa和P. densiflora提取物。在本研究中，挥发性有机化合物（VOCs）的C。obtusa和P. densiflora进行检查，以确定它们是否具有抗炎能力。目的：研究冬虫夏草挥发性有机化合物的抗炎作用。obtusa和P. densiflora，脂多糖（LPS）通过鼻腔注射施用至肺，通过腹膜内注射施用至全身。采用ELISA法检测血清免疫球蛋白E（IgE）和前列腺素E2（PgE 2）水平的变化。LPS升高的血清IgE和PgE 2水平通过给予地塞米松和C. obtusa和密花P. densiflora。在LPS诱导的炎症小鼠模型中测定炎性细胞因子的mRNA表达水平。采用逆转录-定量聚合酶链反应检测外周血单个核细胞中环氧合酶2、白细胞介素（IL）-1、肿瘤坏死因子（TNF）-α和IL-13的mRNA表达水平。地塞米松和C. obtusa和密花P. densiflora。在通过支气管肺泡灌洗获得的肺组织和细胞中观察到类似的趋势。本研究结果表明，C。obtusa和P. densiflora通过它们的免疫抑制活性可能在治疗或预防炎症中具有治疗潜力。
Chamaecyparis obtusa (C. obtusa) and Pinus densiflora (P. densiflora) have been traditionally used as antibiotic, antinociceptive and anti-inflammatory agents in Asian folk medicine. Recent studies have demonstrated antioxidant, antiproliferative and anti-inflammatory effects of C. obtusa and P. densiflora extracts. In the present study, volatile organic compounds (VOCs) of C. obtusa and P. densiflora were examined to determine whether they have anti-inflammatory capabilities. To evaluate the anti-inflammatory effects of VOCs of C. obtusa and P. densiflora, lipopolysaccharide (LPS) was administered to the lung by nasal injection and to the whole body by intraperitoneal injection. Alterations in serum immunoglobulin E (IgE) levels and prostaglandin E2 (PgE2) were examined using ELISA. LPS-increased serum IgE and PgE2 levels were recovered by administration of dexamethasone and VOCs of C. obtusa and P. densiflora. Levels of mRNA expression of inflammatory cytokines were determined in an LPS-induced inflammation mouse model. Reverse transcription-quantitative polymerase chain reaction was used to determine the mRNA expression levels of cyclooxygenase 2, interleukin (IL)-1, tumor necrosis factor (TNF)-alpha and IL-13 in peripheral blood mononuclear cells. The expression of all examined cytokine mRNAs increased by LPS was suppressed by dexamethasone and VOCs of C. obtusa and P. densiflora. Similar tendencies were observed in lung tissues and cells obtained via bronchoalveolar lavage. The results of the present study suggested that VOCs of C. obtusa and P. densiflora, through their immunosuppressive activities, may have therapeutic potential in the treatment or prevention of inflammation.