Curcuma longa inhibits TNF-α induced expression of adhesion molecules on human umbilical vein endothelial cells
Curcuma longa inhibits TNF-α induced expression of adhesion molecules on human umbilical vein endothelial cells
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DOI：
10.1016/s0192-0561(99)00050-8
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发表时间：
1999-11-01
期刊：
影响因子：
--
通讯作者：
Ghosh, B
中科院分区：
文献类型：
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作者：
Gupta, B;Ghosh, B
非甾体类抗炎小分子化合物的鉴定对抗炎药物的开发具有重要意义。我们在此证明，在姜黄乙酸乙酯提取物中存在的三种化合物，即二阿魏酰甲烷、对香豆酰阿魏酰甲烷和二对香豆酰甲烷中，二阿魏酰甲烷在抑制TNF-α诱导的人脐静脉内皮细胞上ICAM-1、VCAM-1和E-选择素表达方面最有效。二阿魏酰甲烷的抑制作用是时间依赖性的，并且是可逆的。通过使用RT-PCR，我们证明它抑制ICAM-1，VCAM-1和E-selectin的稳态转录水平的诱导，因此它可能干扰它们的基因的转录。由于二阿魏酰甲烷显著阻断细胞因子诱导的白细胞粘附分子的转录水平，它可能在TNF-α诱导的信号传导事件的早期阶段干扰。(C)1999年国际免疫药理学学会。由爱思唯尔科技有限公司出版。保留所有权利。
Identification of non-steroidal anti-inflammatory small molecules is very important for the development of anti-inflammatory drugs. We demonstrate here that out of three compounds, viz diferuloylmethane, p-coumaroylferuloylmethane and di-p-coumaroylmethane, present in the ethyl acetate extract of Curcuma longa, diferuloylmethane is most potent in inhibiting TNF-alpha induced expression of ICAM-1, VCAM-1 and E-selectin on human umbilical vein endothelial cells. The inhibition by diferuloylmethane is time dependent and is reversible. By using RT-PCR, we demonstrate that it inhibits the induction of steady state transcript levels of ICAM-1, VCAM-1 and E-selectin, and therefore it may interfere with the transcription of their genes. As diferuloylmethane significantly blocks the cytokine induced transcript levels for the leukocyte adhesion molecules, it may be interfering at an early stage of signalling event induced by TNF-alpha. (C) 1999 International Society for Immunopharmacology. Published by Elsevier Science Ltd. All rights reserved.