A pedigree-based study of mitochondrial D-loop DNA sequence variation among Arabian horses
A pedigree-based study of mitochondrial D-loop DNA sequence variation among Arabian horses
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DOI：
10.1046/j.1365-2052.2000.00558.x
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发表时间：
2000-02-01
期刊：
影响因子：
2.4
通讯作者：
Bowling, M
中科院分区：
文献类型：
--
作者：
Bowling, AT;Del Valle, A;Bowling, M
通过线粒体D-loop高变区的DNA序列比较，我们调查了美国阿拉伯马的母系多样性。对62匹马进行了测试。从已公布的母系谱系，他们追溯到34匹母马，主要是在19世纪中后期从游牧的贝都因部落获得的。与参考序列(GenBank X79547)比较，共发现27种单倍型，共31个碱基替换位点，序列长度为397bp。基于对美国阿拉伯马登记处当前学习书籍中200匹马的随机抽样的系谱检查，我们估计这项研究定义了至少89%在美国注册的阿拉伯马的预期mtDNA单倍型。通过14个品系的多个样本的单倍型一致性，证明了这本教科书记录的阿拉伯家系母系的可靠性。在两个母系中观察到的单碱基差异被解释为代表过去异质性的替代固定。这项研究还证明了mtDNA序列研究在解决历史生育问题方面的作用，而不需要从有问题的马那里获得生物材料，前提是相关雌性品系的代表可以进行比较。这些数据质疑了传统的假设，即相同品系的阿拉伯马必然拥有共同的母系祖先。
Through DNA sequence comparisons of a mitochondrial D-loop hypervariable region, we investigated matrilineal diversity for Arabian horses in the United States. Sixty-two horses were tested. From published pedigrees they traced in the maternal line to 34 mares acquired primarily in the mid to late 19th century from nomadic Bedouin tribes. Compared with the reference sequence (GenBank X79547), these samples showed 27 haplotypes with altogether 31 base substitution sites within 397 bp of sequence. Based on examination of pedigrees from a random sampling of 200 horses in current studbooks of the Arabian Horse Registry of America, we estimated that this study defined the expected mtDNA haplotypes for at least 89% of Arabian horses registered in the US. The reliability of the studbook recorded maternal lineages of Arabian pedigrees was demonstrated by haplotype concordance among multiple samplings in 14 lines. Single base differences observed within two maternal lines were interpreted as representing alternative fixations of past heteroplasmy. The study also demonstrated the utility of mtDNA sequence studies to resolve historical maternity questions without access to biological material from the horses whose relationship was in question, provided that representatives of the relevant female lines were available for comparison. The data call into question the traditional assumption that Arabian horses of the same strain necessarily share a common maternal ancestry.