New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C
New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C
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DOI：
10.1016/j.bbrc.2008.04.005
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发表时间：
2008-06-20
影响因子：
3.1
通讯作者：
Kato, Nobuyuki
中科院分区：
文献类型：
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作者：
Mori, Kyoko;Abe, Ken-ichi;Kato, Nobuyuki
我们首次报道了一种新的 RNA 复制系统，其具有源自急性丙型肝炎患者的丙型肝炎病毒 (HCV) 株 (AHI)。使用由 AH1 株（基因型 1b）构建的 HCV 复制子 RNA 文库，我们首先建立了包含 HCV 复制子的克隆细胞系 sAH1。用干扰素处理sAH1细胞获得的治愈细胞用于转染具有在sAH1复制子中发现的四个突变的基因组长度的HCV RNA。因此，获得了一种支持基因组长度 HCV RNA 有效复制的克隆细胞系 AH1。通过比较支持基因组长度 HCV RNA（HCV-O 株）复制的 AH1 细胞和 O 细胞，我们发现 AH1 和 O 细胞之间干扰素 γ 和环孢素 A 的抗 HCV 谱不同。报告分析表明，干扰素-γ 的不同作用是由于 HCV 毒株的差异造成的，而不是细胞环境的差异。 (c) 2008 Elsevier Inc. 保留所有权利
We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AHI) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-gamma and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-gamma are due to the difference in HCV strains, but not the cellular environment. (c) 2008 Elsevier Inc. All rights reserved