Silk-silica composites from genetically engineered chimeric proteins: materials properties correlate with silica condensation rate and colloidal stability of the proteins in aqueous solution.
Silk-silica composites from genetically engineered chimeric proteins: materials properties correlate with silica condensation rate and colloidal stability of the proteins in aqueous solution.
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DOI：
10.1021/la205084z
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发表时间：
2012-03-06
期刊：
影响因子：
--
通讯作者：
Perry CC
中科院分区：
文献类型：
--
作者：
Belton DJ;Mieszawska AJ;Currie HA;Kaplan DL;Perry CC
该研究的目的是确定一系列已知的硅化重组嵌合体对硅缩合的影响程度和机制（R5- SSKKSGSYSGSKGSKRRIL; A1- SGSKGSKRRIL;和Si 4 -1- MSPHPHPRHHHT及其重复序列）连接在大壶腹拖丝纺锤体蛋白1（Masp 1）的32个氨基酸共有序列的15聚体重复序列的N-末端棒足络新妇蜘蛛丝序列（[SGRGLGGQG AGAAAAAGGA GQGGYGGLGSQG] 15 X）。通过调整硅化结构域的类型和数量（以上表示为X）来探索丝/嵌合体比率的影响，并将结果与它们的非嵌合对应物和来自家蚕的丝进行比较。研究了pH（3-9）对反应活性的影响。确定了二氧化硅沉积速率和控制的最佳条件，并探索了丝的溶液性质以确定其作用模式。对于形成的二氧化硅-丝-嵌合体材料，在嵌合蛋白的溶液性质（携带电荷的能力）、反应的pH和生成的固态材料之间存在关系。胶体不稳定的区域与观察到的形态控制的pH值范围相关，并与最高二氧化硅缩合速率的pH值范围一致。有了这些信息，应该有可能预测嵌合或化学修饰的蛋白质将如何影响在受控条件下生产的材料的结构和形态，并扩展复合材料的范围，用于生物医学和技术领域的广泛用途。
The aim of the study was to determine the extent and mechanism of influence on silica condensation that is presented by a range of known silicifying recombinant chimeras (R5- SSKKSGSYSGSKGSKRRIL; A1- SGSKGSKRRIL; and Si4-1- MSPHPHPRHHHT and repeats thereof) attached at the N-terminus end of a 15 mer repeat of the 32 amino acid consensus sequence of the major ampullate dragline Spindroin 1 (Masp1) Nephila clavipes spider silk sequence ([SGRGGLGGQG AGAAAAAGGA GQGGYGGLGSQG]15X). The influence of the silk/chimera ratio was explored through the adjustment of the type and number of silicifying domains, (denoted X above), and the results were compared with their non chimeric counterparts and the silk from Bombyx mori. The effect of pH (3–9) on reactivity was also explored. Optimum conditions for rate and control of silica deposition were determined and the solution properties of the silks were explored to determine their mode(s) of action. For the silica-silk-chimera materials formed there is a relationship between the solution properties of the chimeric proteins (ability to carry charge), the pH of reaction and the solid state materials that are generated. The region of colloidal instability correlates with the pH range observed for morphological control and coincides with the pH range for the highest silica condensation rates. With this information it should be possible to predict how chimeric or chemically modified proteins will affect structure and morphology of materials produced under controlled conditions and extend the range of composite materials for a wide spectrum of uses in the biomedical and technology fields.
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DOI：
10.1111/j.1432-1033.1990.tb19354.x
发表时间：
1990-10-24
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GOORMAGHTIGH, E;CABIAUX, V;RUYSSCHAERT, JM
通讯作者：
RUYSSCHAERT, JM
影响因子：
4.9
作者：
Knecht, MR;Wright, DW
通讯作者：
Wright, DW
影响因子：
1.7
作者：
Binyamin, Gary;Shafi, Bilal M;Mery, Carlos M
通讯作者：
Mery, Carlos M
影响因子：
2.9
作者：
Chen, Xin;Shao, Zhengzhong;Vollrath, Fritz
通讯作者：
Vollrath, Fritz
影响因子：
6.2
作者：
Kim, UJ;Park, JY;Kaplan, DL
通讯作者：
Kaplan, DL