INVITRO FORMATION OF OOKINETES AND FUNCTIONAL MATURITY OF PLASMODIUM-BERGHEI GAMETOCYTES
INVITRO FORMATION OF OOKINETES AND FUNCTIONAL MATURITY OF PLASMODIUM-BERGHEI GAMETOCYTES
复制标题
DOI：
10.1017/s0031182000056481
复制
发表时间：
1985-01-01
期刊：
影响因子：
2.4
通讯作者：
VANDERKAAY, HJ
中科院分区：
文献类型：
--
作者：
JANSE, CJ;MONS, B;VANDERKAAY, HJ
对伯氏疟原虫动合子的体外形成进行了研究。体外产生的配子体取自瑞士小鼠的心脏和尾血，以及蚊子吸食这些小鼠血液后直接获取的血液，体外产生的配子体还取自伯氏疟原虫红细胞期的短期培养物。使用体内和体外产生的配子体，在RPMI 1640培养基（pH 7.8 - 8.0）中获得了可重复的动合子产生。描述了在光学显微镜下动合子发育阶段的形态以及退化形式。与MEM培养基相比，在RPMI 1640培养基中产生的动合子更多，动合子产量（定义为体外产生的动合子数量/10⁵个红细胞与感染血液中出丝数量/10⁵个红细胞的比率）在测试的稀释范围内随着培养物中红细胞密度的降低而增加。配子体血症与产生的动合子数量之间存在线性关系。体外动合子形成的方法以及动合子产量的估算方法使我们能够研究配子体功能成熟的各个方面，而不受蚊子的影响。尾血中出丝配子体的数量和体外动合子产量与心脏血液以及蚊子吸食的血液中的情况相符，这表明功能成熟的配子体在脊椎动物宿主内是随机分布的。
In vitro formation of P. berghei ookinetes was studied. Gametocytes produced in vitro were obtained from heart and tail blood of Swiss mice and from blood removed from mosquitoes directly after feeding on these mice gametocytes produced in vitro were obtained from short-term cultures of the erythrocytic stages of P. berghei. Reproducible ookinete production was obtained in medium RPMI 1640, pH 7.8-8.0, using in vivo and in vitro produced gametocytes. The morphology of developmental stages of ookinetes and degenerate forms at the light microscope level is described. More ookinetes were produced in medium RPMI 1640 compared to MEM and ookinete yield, defined as the ratio between the number of in vitro produced ookinetes/105 erythrocytes and the number of exflagellations/105 erythroyctes in the infected blood, increased with lower erythrocyte densities in the cultures within the range of dilutions tested. A linear relationship existed between gametocytemia and the number of ookinetes produced. The methods for in vitro ookinete formation and for estimating ookinete yields enabled us to study aspects of functional maturity of gametocytes independent of mosquitoes. The numbers of exflagellating gametocytes and in vitro ookinete yields in tail blood corresponded with those in heart blood and blood ingested by mosquitoes, suggesting a random distribution of functionally mature gametocytes within the vertebrate host.