A growth factor-repressible gene associated with protein kinase C-mediated inhibition of adipocyte differentiation.
A growth factor-repressible gene associated with protein kinase C-mediated inhibition of adipocyte differentiation.
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DOI：
10.1083/jcb.107.1.279
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发表时间：
1988-07
期刊：
影响因子：
--
通讯作者：
Ringold GM
中科院分区：
文献类型：
--
作者：
Navre M;Ringold GM
将确定的成脂肪细胞转化为完全分化的脂肪细胞需要适当的环境条件。先前已经描述了对细胞汇合的严格依赖和糖皮质激素的促进作用。我们已经发现，能够激活蛋白激酶C的试剂，例如碱性成纤维细胞生长因子和佛波醇酯，抑制脂肪形成细胞系TA1的分化而不刺激细胞生长。在这里，我们描述了检测RNA的cDNA（克隆5）的序列和表征，其表达通过糖皮质激素和增加细胞密度而增强。相反，蛋白激酶C的激活剂包括碱性成纤维细胞生长因子、佛波醇酯和合成二酰基甘油抑制克隆5基因表达。克隆5的表达似乎与促进成脂细胞分化的环境和激素因素密切相关。
The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating protein kinase C, such as basic fibroblast growth factor and phorbol esters, inhibit the differentiation of the adipogenic cell line TA1 without stimulating cell growth. Here we describe the sequence and characterization of a cDNA (clone 5) that detects an RNA, the expression of which is enhanced by glucocorticoids and increasing cell density. In contrast, activators of protein kinase C including basic fibroblast growth factor, phorbol esters, and synthetic diacylglycerols inhibit clone 5 gene expression. It appears that clone 5 expression is closely linked to environmental and hormonal factors that promote the differentiation of adipogenic cells.