A system for site-specific genetic manipulation of the relapsing fever spirochete Borrelia hermsii.
A system for site-specific genetic manipulation of the relapsing fever spirochete Borrelia hermsii.
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回归热螺旋体赫氏疏螺旋体的位点特异性基因操作系统。
DOI：
10.1007/978-1-60327-032-8_6
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Schwan,TomG
中科院分区：
文献类型：
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作者：
Battisti,JamesM;Raffel,SandraJ;Schwan,TomG
缺乏一个系统的遗传操作阻碍了研究的分子发病机制复发热疏螺旋体。本章的重点是描述选择性标记，操作策略和方法，电转化和克隆野生型感染性赫氏疏螺旋体。初步研究表明，B.涉及蜱虫向哺乳动物传播的赫姆斯虫。为了解决这一假设，我们已经开发了一个系统的遗传操作，并构建了一个VTP突变体和同基因重组菌株的克隆。本文所述的方法也适用于B中其它基因座的失活。hermsiiand应该适用于其他种类的回归热螺旋体。
The lack of a system for genetic manipulation has hindered studies on the molecular pathogenesis of relapsing feverBorrelia. The focus of this chapter is to describe selectable markers, manipulation strategies, and methods to electro-transform and clone wild-type infectiousBorrelia hermsii. Preliminary studies suggest that thevariabletickprotein (Vtp) ofB. hermsiiis involved in tick-to-mammal transmission. To address this hypothesis, we have developed a system for genetic manipulation and have constructed clones of a Vtp mutant and an isogenic reconstituted strain. The methods described here are applicable for the inactivation of other loci inB. hermsiiand should be adaptable for other species of relapsing fever spirochetes.