Vaping product exposure system (VaPES): a novel in vitro aerosol deposition system.
Vaping product exposure system (VaPES): a novel in vitro aerosol deposition system.
复制标题
电子烟产品暴露系统（VaPES）：一种新型体外气溶胶沉积系统。
DOI：
10.1080/08958378.2023.2289021
复制
发表时间：
2023
影响因子：
2.1
通讯作者：
Jaspers,Ilona
中科院分区：
文献类型：
--
作者：
Schichlein,KevinD;Love,CharlotteA;Conolly,MaxwellP;Kurz,JohnL;Hickman,EliseD;Clapp,PhillipW;Jaspers,Ilona
由于最近电子烟设备的使用增加，对解决电子烟产品对呼吸健康影响的研究需求很高。鉴于电子烟市场的性质不断变化，新的装置、香料、金属和其他化学品迅速出现，因此需要廉价且适应性强的电子烟装置暴露系统。在这里，我们描述的设计和验证的novelin vitroaerosol暴露系统的毒性测试的vaping devices.Materials and methodsWe开发了一种廉价的，开源的vitrovaping设备暴露系统，产生均匀的沉积，可以适用于不同的vaping设备，并允许在生理条件下进行实验。然后，该系统进行了验证与沉积测试和代表性的暴露与人支气管上皮细胞（hBECs）。ResultsThe Vaping产品暴露系统（VaPES）产生足够的和均匀的沉积剂量-反应研究，并足够精确，以观察生物反应vaping曝光。VaPES是适应工作与两个吊舱和基于电子烟device.ConclusionWe已经设计并验证了一种新型的电子烟设备暴露系统，这将消除需要使用高成本的商业暴露系统，降低进入生理相关的电子烟研究的障碍。
ObjectiveDue to recent increases in the use of vaping devices, there is a high demand for research addressing the respiratory health effects of vaping products. Given the constantly changing nature of the vaping market with new devices, flavors, metals, and other chemicals rapidly emerging, there is a need for inexpensive and highly adaptable vaping device exposure systems. Here, we describe the design and validation of a novelin vitroaerosol exposure system for toxicity testing of vaping devices.Materials and methodsWe developed an inexpensive, open-sourcein vitrovaping device exposure system that produces even deposition, can be adapted for different vaping devices, and allows for experiments to be performed under physiological conditions. The system was then validated with deposition testing and a representative exposure with human bronchial epithelial cells (hBECs).ResultsThe Vaping Product Exposure System (VaPES) produced sufficient and uniform deposition for dose-response studies and was precise enough to observe biological responses to vaping exposures. VaPES was adapted to work with both pod and cartridge-based vaping devices.ConclusionWe have designed and validated a novel vaping device exposure system that will eliminate the need to use high-cost commercial exposure systems, lowering the barrier to entry of physiologically relevant vaping studies.