Adenovirus 12S E1A gene represses differentiation of F9 teratocarcinoma cells.
Adenovirus 12S E1A gene represses differentiation of F9 teratocarcinoma cells.
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腺病毒 12S E1A 基因抑制 F9 畸胎癌细胞的分化。
DOI：
10.1073/pnas.87.24.9878
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发表时间：
1990
影响因子：
11.1
通讯作者：
Nevins,JR
中科院分区：
文献类型：
--
作者：
Weigel,RJ;Devoto,SH;Nevins,JR
F9 畸胎癌细胞系在用视黄酸和 cAMP 处理后在体外分化，并且已成为广泛使用的模型系统，用于研究早期发育过程中负责细胞定型和分化的分子事件。先前的实验表明F9细胞分化过程中基因表达的控制与腺病毒E1A对基因表达的调节之间存在有趣的相似之处。将表达 12S E1A 的质粒转染至终末分化、非增殖的 F9 细胞中，会以高频率产生分裂细胞集落，每个细胞都表达 E1A。从这些集落建立的细胞系在视黄酸存在下增殖，并失去了完全分化的表型，其特征是缺乏一系列分化特异性基因的表达。我们得出结论，病毒 12S E1A 基因产物的表达会干扰视黄酸诱导的 F9 细胞分化。此外，结果表明，由终末分化标记定义的分化过程可能不是永久事件，但可以通过 E1A 表达来逆转。
The F9 teratocarcinoma cell line differentiates in vitro after treatment with retinoic acid and cAMP and has been a widely used model system for the study of the molecular events that are responsible for cellular commitment and differentiation during early development. Previous experiments have suggested intriguing parallels between the control of gene expression during F9 cell differentiation and the regulation of gene expression by adenovirus E1A. Transfection of a 12S E1A-expressing plasmid into terminally differentiated, nonproliferating F9 cells generates, at high frequency, colonies of dividing cells, each of which expresses E1A. Cell lines established from these colonies proliferate in the presence of retinoic acid and have lost the fully differentiated phenotype as characterized by the absence of expression of a series of differentiation-specific genes. We conclude that expression of the viral 12S E1A gene product interferes with retinoic acid-induced F9 cell differentiation. Moreover, the results suggest that the differentiation process, as defined by markers of terminal differentiation, may not be a permanent event but can be reversed by E1A expression.