Age-related increases in plasma phosphatidylcholine hydroperoxide concentrations in control subjects and patients with hyperlipidemia.
Age-related increases in plasma phosphatidylcholine hydroperoxide concentrations in control subjects and patients with hyperlipidemia.
复制标题
对照受试者和高脂血症患者的血浆磷脂酰胆碱过氧化氢浓度与年龄相关。
DOI：
10.1093/clinchem/46.6.822
复制
发表时间：
2000
影响因子：
9.3
通讯作者：
T. Miyazawa
中科院分区：
文献类型：
--
作者：
M. Kinoshita;S. Oikawa;K. Hayasaka;A. Sekikawa;Tazuko Nagashima;T. Toyota;T. Miyazawa
背景
高脂血症患者血浆基础过氧化脂质浓度可能与动脉粥样硬化有关。血浆过氧化脂质的定量测定是全面评价氧化损伤生化过程的重要步骤。遗憾的是，目前可用的检测脂质过氧化的方法缺乏特异性和敏感性。
方法
高脂血症患者44例，女性50例，年龄12~82岁(平均+/-2.3岁，男性53+/-2.3岁，女性58+/-2.0岁，总病例56+/-14岁)和正常血脂志愿者(对照组，男性32例，女性15例)，年龄13-90岁(男性49+/-4岁，女性65+/-4岁，总病例55+/-24岁)。采用化学发光法测定血浆中过氧化氢磷脂酰胆碱(PCOOH)含量。
结果
对照组和高脂血症患者的血浆PCOOH浓度均随年龄增长而升高。而高脂血症患者血浆PCOOH平均浓度(331+/-19nmol/L；n=94)显著高于对照组(160+/-65nmol/L；n=47)(P<0.001)。三种高脂血症表型：高胆固醇血症(IIa)、高甘油三酯血症(IV)和混合性高脂血症(IIb)的血浆PCOOH浓度相似。治疗后血脂正常者平均血浆PCOOH为202+/-17nmol/L。高脂血症患者血浆PCOOH浓度与总胆固醇、甘油三酯、磷脂无明显相关性。在所有受试者中，血浆PCOOH与各种血脂呈显著正相关(总胆固醇，P=0.0002；甘油三酯，P=0.0137；磷脂，P<；0.0001)。血浆磷脂酰胆碱的脂肪酸组成分析显示，与对照组相比，患者的n-6脂肪酸部分(亚油酸和花生四烯酸)浓度显著降低。
结论
我们的结果提示，高脂血症患者血浆PCOOH的升高可能与动脉粥样硬化的发生和发展有关，尤其是在老年人。血浆PCOOH的测定对体内氧化应激的评估是有用的。
BACKGROUND
The basal lipid peroxide concentration in the plasma of patients with hyperlipidemia may be related to atherosclerosis. Quantitative determination of lipid peroxides in the plasma is an important step in the overall evaluation of the biochemical processes leading to oxidative injury. Unfortunately, the currently available methods for lipid peroxidation lack specificity and sensitivity.
METHODS
Hyperlipidemic patients (44 males and 50 females), ages 12-82 years (mean +/- SE, 53 +/- 2.3 years for males, 58 +/- 2.0 years for females, and 56 +/- 14 years for total cases), and normolipidemic volunteers (controls, 32 males and 15 females), ages 13-90 years (49 +/- 4 years for males, 65 +/- 4 years for females, and 55 +/- 24 years for total cases), were recruited in the present study. Plasma phosphatidylcholine hydroperoxide (PCOOH) was determined by chemiluminescence-HPLC (CL-HPLC).
RESULTS
Plasma PCOOH concentrations increased with age in both controls and hyperlipidemic patients. However, the mean plasma PCOOH concentration in patients with hyperlipidemia (331 +/- 19 nmol/L; n = 94) was significantly (P <0.001) higher than in the controls (160 +/- 65 nmol/L; n = 47). Plasma PCOOH concentrations were similar in three hyperlipidemic phenotypes: hypercholesterolemia (IIa), hypertriglyceridemia (IV), and combined hyperlipidemia (IIb). The mean plasma PCOOH in patients with treatment-induced normalized plasma lipids was 202 +/- 17 nmol/L. There was no significant correlation between plasma PCOOH concentration and total cholesterol, triglycerides, or phospholipids in hyperlipidemic patients. For all subjects, there was a significantly positive correlation between plasma PCOOH and each lipid (total cholesterol, P = 0.0002; triglycerides, P = 0.0137; and phospholipids, P <0.0001). Analysis of fatty acids composition of plasma phosphatidylcholine showed significantly low concentrations of n-6 fatty acids moieties (linoleic acid and arachidonic acid) in patients compared with controls.
CONCLUSIONS
Our results suggest that an increase in plasma PCOOH in patients with hyperlipidemia may be related to the development and progression of atherosclerosis, particularly in the elderly. Measurement of plasma PCOOH is useful for in vivo evaluation of oxidative stress.
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DOI：
10.1073/pnas.84.9.2995
发表时间：
1987-05-01
影响因子：
11.1
作者：
QUINN, MT;PARTHASARATHY, S;STEINBERG, D
通讯作者：
STEINBERG, D
DOI：
10.1073/pnas.81.12.3883
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
STEINBRECHER, UP;PARTHASARATHY, S;STEINBERG, D
通讯作者：
STEINBERG, D
超氧化物引发人单核细胞对低密度脂蛋白的氧化。
DOI：
10.1161/01.atv.7.1.55
发表时间：
1987
期刊：
Arteriosclerosis (Dallas, Tex.)
影响因子：
--
作者：
Hiramatsu,K;Rosen,H;Heinecke,JW;Wolfbauer,G;Chait,A
通讯作者：
Chait,A
影响因子：
6.5
作者：
S. Rankin;S. Parthasarathy;D. Steinberg
通讯作者：
S. Rankin;S. Parthasarathy;D. Steinberg
富含甘油三酯的脂蛋白和动脉粥样硬化：病理生理学考虑。
DOI：
--
发表时间：
1994
期刊：
Journal of internal medicine. Supplement
影响因子：
--
作者：
Bradley,WA;Gianturco,SH
通讯作者：
Gianturco,SH