Inflammatory profiles in nasal mucosa of patients with persistent vs intermittent allergic rhinitis
Inflammatory profiles in nasal mucosa of patients with persistent vs intermittent allergic rhinitis
复制标题
持续性与间歇性过敏性鼻炎患者鼻粘膜炎症特征
DOI：
10.1111/j.1398-9995.2010.02340.x
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发表时间：
2010-09-01
期刊：
影响因子：
12.4
通讯作者：
Bachert, C.
中科院分区：
文献类型：
--
作者：
Liu, F.;Zhang, J.;Bachert, C.
背景：迄今为止，关于持续性（PER）和间歇性变应性鼻炎（IAR）患者的炎症特征的信息很少。此外，尚不清楚嗜酸性炎症和/或t辅助细胞亚群及其标记物是否存在差异。本研究的目的主要是评估中度/重度PER和IAR患者的炎症特征。方法：选取12例PER、12例IAR和12例非过敏性非鼻炎（对照组）患者的下鼻甲组织，监测症状（视觉模拟量表、VAS）和生活障碍。采用免疫组化法检测各组组织嗜酸性粒细胞和肥大细胞数量；免疫分析法检测IL-5、ECP、IgE浓度；实时定量聚合酶链反应检测转录因子GATA-3、T-bet、FOXP3和RORc的mRNA；以及ige诱导的LTC4/D-4/E-4和PGD的体外释放(2)。结果：与对照组相比，PER和IAR患者嗜酸性粒细胞和肥大细胞明显增加；与IAR患者相比，PER患者两种细胞类型的增加更为显著。同样，与IAR患者相比，PER患者的ECP IL-5、GATA-3 mRNA表达和ige诱导的肥大细胞LTC4/D-4/E-4和PGD(2)的释放显著增加。相比之下，T-bet、FOXP3和RORc mRNA的表达在PER、IAR和对照组中无显著差异。结论：本研究的结果表明，与IAR相比，PER具有明显更大的嗜酸性粒细胞和主要由Th2细胞介导的鼻腔炎症特征。
Background: To date there is little information on the inflammatory profiles of patients suffering from persistent (PER) and intermittent allergic rhinitis (IAR). Also, it is not clear whether differences exist in eosinophilic inflammation and/or T-helper cell sub-populations and their markers. The aim of this study was to primarily evaluate the inflammatory profiles of patients with moderate/severe PER and IAR.Methods: Inferior nasal turbinate tissue was obtained from 12 PER, 12 IAR and 12 nonallergic nonrhinitic (control) patients, and symptoms (visual analogue scales, VAS) and impairment of life was monitored. All tissues were assessed for eosinophil and mast cell numbers by immunohistochemistry; IL-5, ECP and IgE concentrations by immunoassay; mRNA for transcription factors GATA-3, T-bet, FOXP3 and RORc by quantitative real-time polymerase chain reaction; and IgE-induced release of LTC4/D-4/E-4 and PGD(2) in vitro.Results: Eosinophils and mast cells were significantly increased in patients with PER and patients with IAR compared to control subjects; by patients with PER demonstrating even significantly greater increase of both cell types than patients with IAR. Similarly, ECP IL-5, GATA-3 mRNA expression and IgE-induced release of LTC4/D-4/E-4 and PGD(2) from mast cells were significantly increased in patients with PER compared to patients with IAR. In contrast, the expression of T-bet, FOXP3 or RORc mRNA was not significantly different in the PER, IAR or control patients.Conclusion: The findings from the present study suggest that PER is characterized by a significantly greater eosinophilic and predominantly Th2 cell-mediated nasal inflammatory profile compared to IAR.