Preparation of viable tumour cell vaccine from human solid tumours: relationship between tumour mass and cell yield. The Tissue Bank, Pittsburgh Cancer Institute.
Preparation of viable tumour cell vaccine from human solid tumours: relationship between tumour mass and cell yield. The Tissue Bank, Pittsburgh Cancer Institute.
复制标题
从人类实体瘤制备活肿瘤细胞疫苗：肿瘤质量与细胞产量之间的关系。
DOI：
10.1097/00008390-199311000-00008
复制
发表时间：
1993
影响因子：
2.2
通讯作者：
Straw,LD
中科院分区：
文献类型：
--
作者：
Logan,TF;Shannon,W;Bryant,J;Kane,P;Wolmark,N;Posner,M;Kirkwood,JM;Ernstoff,MS;Futrell,JW;Straw,LD
癌症的主动特异性免疫治疗通常需要使用自体或异体肿瘤细胞作为免疫抗原。在这种方案下获得了肿瘤。然而，预处理的新鲜肿瘤肿块的活细胞产量的估计是困难的，并且最初似乎没有在预处理的肿瘤肿块和处理后获得的活细胞之间的直接关系。因此，我们分析了所有293个肿瘤标本，试图辨别这种关系。其中黑色素瘤137例，肉瘤14例，腺癌48例，肾细胞癌59例，其他35例。预处理肿瘤质量与活细胞产量呈正相关，Spearman相关值从r= 0.49（腺癌）到r= 0.84（黑色素瘤）不等。所有肿瘤的Spearman相关性为r= 0.70 （p= 0.0001）。不出所料，与细菌污染相关的最常见切除部位是肠道。总之，这项研究提供了有用的曲线预测活的肿瘤细胞产率从预处理肿瘤肿块给定的组织学。
Active specific Immunotherapy for cancer often requires the use of autologous or allogeneic tumour cells as Immunizing antigen. Tumours were obtained for such a protocol. However, estimation of viable cell yield from pre-processed fresh tumour mass was difficult, and Initially there did not appear to be a direct relationship between pre-processed tumour mass and viable cells obtained after processing. We therefore analysed all of 293 tumour specimens processed to attempt to discern such a relationship. Of these 137 were melanoma, 14 were sarcoma, 48 were adenocarcinoma, 59 were renal cell carcinoma and 35 were classified as other. A positive correlation was found between pre-processed tumour mass and viable cell yield, with Spearman correlation values varying from r= 0.49 (adenocarcinoma) to r= 0.84 (melanoma). For all tumours the Spearman correlation was r= 0.70 (p= 0.0001). Not surprisingly, the most frequent site of removal associated with bacterial contamination was bowel. In conclusion, this study provides useful curves for predicting viable tumour cell yield from pre-processed tumour mass of given histology.