Role of the blood-brain barrier in the formation of long-chain omega-3 and omega-6 fatty acids from essential fatty acid precursors.
Role of the blood-brain barrier in the formation of long-chain omega-3 and omega-6 fatty acids from essential fatty acid precursors.
复制标题
血脑屏障在从必需脂肪酸前体形成长链 omega-3 和 omega-6 脂肪酸中的作用。
DOI：
10.1111/j.1471-4159.1990.tb04150.x
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发表时间：
1990
影响因子：
4.7
通讯作者：
Spector,AA
中科院分区：
文献类型：
--
作者：
Moore,SA;Yoder,E;Spector,AA
细长的亚油酸(18：2ω-6)和亚麻酸(18：3ω-3)的多不饱和衍生物在大脑中积累，但它们的合成部位和进入机制尚未得到很好的描述。为探讨血脑屏障在此过程中的作用，将培养的小鼠脑微血管内皮细胞与[1-14C]18：2ω-6或[1-14C]18：3ω-3孵育，测定其伸长/去饱和产物。18：2ω-6的主要代谢产物为20：4ω-6，而18：3ω-3的主要代谢产物为20：5ω-3。虽然这些产物主要存在于细胞脂中，但它们也会从细胞中释放出来，并逐渐积累在细胞外液中。从形成的20：4ω-6和20：5ω-3中观察到二十烷类化合物的产生。没有检测到22：5ω-6或22：6ω-3脂肪酸，这表明这些内皮细胞不是最终去饱和步骤的位置。虽然18：3ω-3和18：2ω-6的摄取几乎相同，但18：3ω-3更广泛地延长和去饱和。竞争实验表明，18：3的ω-3通过伸长/去饱和途径偏爱。这些发现表明，血脑屏障在ω-3和ω-6必需脂肪酸从循环转移到大脑的过程中可能在延长和降低饱和度方面发挥重要作用。
Elongated, more highly polyunsaturated derivatives of linoleic acid (18:2ω‐6) and linolenic acid (18:3ω‐3) accumulate in brain, but their sites of synthesis and mechanism of entry are not well characterized. To investigate the role of the blood‐brain barrier in this process, cultured murine cere‐bromicrovascular endothelia were incubated with [1‐14C]18: 2ω‐6 or [1‐14C]18:3ω‐3 and their elongation/desaturation products determined. The major metabolite of 18:2ω‐6 was 20:4ω‐6, whereas the primary product from 18:3ω‐3 was 20: 5ω‐3. Although these products were found primarily in cell lipids, they were also released from the cells and gradually accumulated in the extracellular fluid. Eicosanoid production was observed from the 20:4ω‐6 and 20:5ω‐3 that were formed. No 22:5ω‐6 or 22:6ω‐3 fatty acids were detected, suggesting that these endothelial cells are not the site of the final desaturation step. Although the uptake of 18:3ω‐3 and 18:2ω‐6 was nearly identical, 18:3ω‐3 was more extensively elongated and desaturated. Competition experiments demonstrated a preference for 18:3ω‐3 by the elongation/desaturation pathway. These findings suggest that the blood‐brain barrier can play an important role in the elongation and desaturation of ω‐3 and ω‐6 essential fatty acids during their transfer from the circulation into the brain.