Transmembrane orientation and topology of the NADH:quinone oxidoreductase putative quinone binding subunit NuoH.
Transmembrane orientation and topology of the NADH:quinone oxidoreductase putative quinone binding subunit NuoH.
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NADH：醌氧化还原酶推定醌结合亚基 NuoH 的跨膜方向和拓扑。
DOI：
10.1016/s0005-2728(00)00265-6
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
C. Hägerhäll
中科院分区：
文献类型：
--
作者：
Robert S. Roth;C. Hägerhäll
NADH：醌氧化还原酶，或复合物I，是许多原核生物和真核生物呼吸链中的多亚基膜结合酶。该酶催化NADH的氧化并向醌池提供电子，与质子跨膜转运偶联，但能量转导的机制尚不清楚。在细菌中，这种酶由14个亚基组成，其中7个跨膜，7个从膜上突出。疏水性NuoH（NQO 8，ND 1，NAD 1，NdhA）亚基似乎参与醌结合。在F420 H2氧化还原酶和复杂的膜结合氢化酶中也发现了一个同源的、结构上和功能上相似的亚基。我们对NuoH和NuoH样多肽进行了理论分析，并通过构建和分析碱性磷酸酶融合蛋白，对荚膜红细菌NuoH亚基的跨膜拓扑结构进行了实验分析。这表明NuoH多肽具有八个跨膜片段和四个面向细菌细胞质内部的高度保守的亲水序列基序。N-末端和C-末端位于膜的外侧。基于这些结果的拓扑模型的NuoH，并讨论了该模型的影响。
NADH:quinone oxidoreductase, or Complex I, is a multi-subunit membrane-bound enzyme in the respiratory chain of many pro- and eukaryotes. The enzyme catalyzes the oxidation of NADH and donates electrons to the quinone pool, coupled to proton translocation across the membrane, but the mechanism of energy transduction is not understood. In bacteria the enzyme consists of 14 subunits, seven membrane spanning and seven protruding from the membrane. The hydrophobic NuoH (NQO8, ND1, NAD1, NdhA) subunit is seemingly involved in quinone binding. A homologous, structurally and most likely functionally similar subunit is also found in F420H2oxidoreductases and in complex membrane-bound hydrogenases. We have made theoretical analyses of NuoH and NuoH-like polypeptides and experimentally analyzed the transmembrane topology of the NuoH subunit from Rhodobacter capsulatus by constructing and analyzing alkaline phosphatase fusion proteins. This demonstrated that the NuoH polypeptide has eight transmembrane segments, and four highly conserved hydrophilic sequence motifs facing the inside, bacterial cytoplasm. The N-terminal and C-terminal ends are located on the outside of the membrane. A topology model of NuoH based on these results is presented, and implications from the model are discussed.
使用基因融合来检查球形红杆菌光合反应中心 L 亚基和 bc1 复合体细胞色素 b 亚基的膜拓扑结构。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yun,CH;VanDoren,SR;Crofts,AR;Gennis,RB
通讯作者：
Gennis,RB
DOI：
10.1111/j.1432-1033.1994.tb19985.x
发表时间：
1994-01-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FRIEDRICH, T;VANHEEK, P;WEISS, H
通讯作者：
WEISS, H
影响因子：
56.9
作者：
LAWRENCE, CE;ALTSCHUL, SF;WOOTTON, JC
通讯作者：
WOOTTON, JC