ANTIVIRAL ACTIVITY OF ANTISERUM SPECIFIC FOR AN INFLUENZA VIRUS NEURAMINIDASE
ANTIVIRAL ACTIVITY OF ANTISERUM SPECIFIC FOR AN INFLUENZA VIRUS NEURAMINIDASE
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DOI：
10.1128/jvi.2.4.281-288.1968
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发表时间：
1968-01-01
影响因子：
5.4
通讯作者：
WEBSTER, RG
中科院分区：
文献类型：
--
作者：
KILBOURNE, ED;LAVER, WG;WEBSTER, RG
通过用纯化的酶免疫家兔来产生特异针对流感 A2 神经氨酸酶的抗血清，该酶是通过电泳从去污剂破坏的 A0A2 流感病毒重组体 [X-7 (F1)] 的蛋白质中分离出来的。该重组体含有 A0 亚型血凝素和 A2 神经氨酸酶。在对缺乏 A2 酶的 A0 或 A2 亚型病毒进行的四项血清学测试中，针对分离的 A2 神经氨酸酶的抗血清均未发生反应。相反，抗血清仅抑制含有A2酶的野生型和重组病毒的神经氨酸酶活性，无论其血凝素蛋白的性质如何。抗血清对一些（但不是全部）带有 A2 酶的病毒造成血凝抑制，并且它减少了所有测试的含有 A2 神经氨酸酶的病毒的噬菌斑大小或噬菌斑数量。在鸡胚和细胞培养物中，低浓度的抗血清会降低病毒的产量。鸡胚中的病毒并未发生真正的中和。我们得出结论，A2神经氨酸酶特异性抗血清影响流感病毒感染细胞中病毒的产量和释放。
Antiserum specific for influenza A2neuraminidase was produced by immunization of rabbits with the purified enzyme which had been isolated by electrophoresis from the proteins of a detergent-disrupted A0A2influenza virus recombinant [X-7 (F1)]. This recombinant contained hemagglutinin of the A0subtype and A2neuraminidase. Antiserum to the isolated A2neuraminidase did not react in any of four serological tests with A0or A2subtype viruses that lacked the A2enzyme. In contrast, the antiserum inhibited the neuraminidase activity only of wild-type and recombinant viruses containing the A2enzyme, regardless of the nature of their hemagglutinin proteins. The antiserum caused hemagglutination-inhibition of some, but not all, viruses bearing the A2enzyme, and it reduced the plaque size or plaque number of all viruses tested that contained A2neuraminidase. In the chick embryo and in cell culture, low dilutions of antiserum reduced the yield of virus. True neutralization of virus in the chick embryo did not occur. We conclude that an antiserum specific for A2neuraminidase influenced the yield and release of virus from influenza virus-infected cells.