StMAPK3 controls oxidase activity, photosynthesis and stomatal aperture under salinity and osmosis stress in potato.
StMAPK3 controls oxidase activity, photosynthesis and stomatal aperture under salinity and osmosis stress in potato.
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DOI：
10.1016/j.plaphy.2020.09.012
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
Xi Zhu;Ning Zhang;Xue Liu;Shulin Wang;Shigui Li;Jiangwei Yang;Fangfang Wang;H. Si
中科院分区：
文献类型：
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作者：
Xi Zhu;Ning Zhang;Xue Liu;Shulin Wang;Shigui Li;Jiangwei Yang;Fangfang Wang;H. Si
丝裂原活化蛋白激酶3（MAPK 3）参与植物的生长发育以及对逆境胁迫的应答。本研究旨在探讨StMAPK 3在盐胁迫和渗透胁迫中的作用。使用聚乙二醇（PEG）（5%和10%）和甘露醇（40 mM和80 mM）诱导渗透胁迫。以40 mM和80 mM NaCl胁迫马铃薯为材料，构建StMAPK3过表达和RNA干扰介导的StMAPK3敲低载体，研究StMAPK3对马铃薯生长、气孔孔径、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性以及H2O2、脯氨酸和丙二醛（MDA）含量的影响。同时测定了蒸腾速率、净光合速率、气孔导度和水分利用效率。还检测了StMAPK3蛋白的亚细胞定位。PEG、甘露醇和NaCl处理诱导马铃薯植株中StMAPK 3mRNA的积累。StMAPK3蛋白定位于细胞膜和细胞核。在渗透胁迫和盐胁迫下，StMAPK3的异常表达改变了马铃薯的表型、SOD、CAT和POD酶活性以及H2O2、脯氨酸和MDA含量。在PEG、甘露醇和NaCl处理下，StMAPK3对马铃薯光合作用和气孔开度均有调节作用。马铃薯表型和生理活性的调节表明StMAPK3作为渗透和耐盐性的调节剂。
Mitogen-activated protein kinase 3 (MAPK3) is involved in plant growth and development, as well as response to adverse stress. Here we aimed to explore the role ofStMAPK3in response to salt and osmosis stress. Polyethylene glycol (PEG) (5% and 10%) and mannitol (40 mM and 80 mM) were used to induce osmosis stress. To induce salinity stress, potato plant was cultured with NaCl (40 mM and 80 mM).StMAPK3overexpression and RNA interference-mediatedStMAPK3knockdown were constructed to explore the role ofStMAPK3in potato growth, stomatal aperture size, activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and contents of H2O2, proline and malonaldehyde (MDA). Meanwhile, we detected transpiration, net photosynthesis, stomatal conductance, and water use efficiency. Subcellular location of StMAPK3 protein was also detected. PEG, mannitol and NaCl treatments induced the accumulation ofStMAPK3mRNA in potato plants. StMAPK3 protein was located on the membrane and nucleus. Abnormal expression ofStMAPK3changed potato phenotypes, enzyme activity of SOD, CAT and POD, as well as H2O2, proline and MDA contents under osmosis and salinity stress. Photosynthesis and stomatal aperture were regulated byStMAPK3in potato treated by PEG, mannitol and NaCl. Modulation of potato phenotypes and physiological activity indicatesStMAPK3as a regulator of osmosis and salinity tolerance.