PHYLOGENY OF SYMBIOTIC BACTERIA OF 4 WEEVIL SPECIES (COLEOPTERA, CURCULIONIDAE) BASED ON ANALYSIS OF 16S RIBOSOMAL DNA
PHYLOGENY OF SYMBIOTIC BACTERIA OF 4 WEEVIL SPECIES (COLEOPTERA, CURCULIONIDAE) BASED ON ANALYSIS OF 16S RIBOSOMAL DNA
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DOI：
10.1016/0965-1748(92)90136-3
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发表时间：
1992-07-01
影响因子：
3.8
通讯作者：
TURNER, CE
中科院分区：
文献类型：
--
作者：
CAMPBELL, BC;BRAGG, TS;TURNER, CE
象甲具有原核共生体，这种共生体存在于特殊的器官状结构--真菌体内。无法在昆虫宿主外培养这些细菌共生体，阻碍了确定它们与其他真细菌的分类联系的努力。在这份报告中，16S核糖体RNA的分子系统发育分析表明，分类上不同的象甲共生体是明显分离的细菌谱系的后代。米象和玉米象的胞内共生体来自同一祖先，与豌豆蚜的次生共生体Pisum(Harris)具有共同的谱系。这些共生体是变形杆菌GAMA-3亚群中肠杆菌科的成员。东方棒象的共生体和锥状犀牛的共生体在系统发育上与共生体中的共生体不同，它们属于α亚纲的一个分支，包括昆虫的细胞质不亲和微生物(CIMS)、埃利希菌和无浆体。讨论了细菌共生体的遗传分化对象甲物种形成的意义。
Weevils possess procaryotic symbiotes harbored in specialized organ-like structures, mycetomes. Inability to culture these bacterial symbiotes outside of the insect-host has hindered efforts to determine their taxonomic affiliation with respect to other eubacteria. In this report, molecular phylogenetic analysis of 16S ribosomal RNAs indicates that symbiotes from taxonomically divergent weevils are descendents of distinctly separate bacterial lineages. The intracellular symbiotes of two rhyncophorine weevils, Sitophilus oryzae L. and Sitophilus zeamais Mots., descended from the same hypothetical ancestor and share a common lineage with the secondary symbiote of the pea aphid, Acyrthosiphon pisum (Harris). These symbiotes are members of the Enterobacteriaceae in the gamma-3 subgroup of the Proteobacteria. The symbiotes of two cleonine weevils, Bangasternus orientalis (Capiomont) and Rhinocyllus conicus (Froelich), are phylogenetically distinct from the symbiotes found in the rhyncophorines and are members of a branch of the alpha-subdivision which include the cytoplasmic incompatibility microorganisms of insects (CIMS), Ehrlichia and Anaplasma. The implication of genetic divergence of bacterial symbiotes to weevil speciation is discussed.