An apicobasal gradient of Rac activity determines protrusion form and position.
An apicobasal gradient of Rac activity determines protrusion form and position.
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DOI：
10.1038/ncomms15385
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发表时间：
2017-05-19
影响因子：
16.6
通讯作者：
Georgiou M
中科院分区：
文献类型：
--
作者：
Couto A;Mack NA;Favia L;Georgiou M
极化上皮层内的每个细胞必须对齐并正确定位广泛的亚细胞结构，包括基于肌动蛋白的动态突起。使用体内可诱导的转基因，可以感测或修改Rac活性，我们证明了一个apicobasal梯度的Rac活性，需要正确地形成和定位不同类别的动态突起沿着apicobasal轴的细胞。我们表明，我们可以修改特定极性蛋白质的遗传突变体中的Rac活性梯度，从而改变突起的形式和位置，并使用可光活化的Rac转基因另外表明，Rac活性水平决定突起的形式。因此，我们证明了一种机制，极性蛋白可以在空间上调节Rac活性和肌动蛋白细胞骨架，以确保正确的上皮细胞形状，并防止上皮间质的转换。极化的上皮细胞必须正确定位广泛的亚细胞结构。在这里，作者证明了一个apicobasal梯度的Rac GTdR活性，这是维持在果蝇上皮片的极性蛋白，并需要保持肌动蛋白依赖性突起的形式和位置。
Each cell within a polarized epithelial sheet must align and correctly position a wide range of subcellular structures, including actin-based dynamic protrusions. Using in vivo inducible transgenes that can sense or modify Rac activity, we demonstrate an apicobasal gradient of Rac activity that is required to correctly form and position distinct classes of dynamic protrusion along the apicobasal axis of the cell. We show that we can modify the Rac activity gradient in genetic mutants for specific polarity proteins, with consequent changes in protrusion form and position and additionally show, using photoactivatable Rac transgenes, that it is the level of Rac activity that determines protrusion form. Thus, we demonstrate a mechanism by which polarity proteins can spatially regulate Rac activity and the actin cytoskeleton to ensure correct epithelial cell shape and prevent epithelial-to-mesenchymal transitions. Polarized epithelial cells must correctly position a wide range of subcellular structures. Here the authors demonstrate an apicobasal gradient of Rac GTPase activity, which is maintained by polarity proteins in Drosophila epithelial sheets, and is required to maintain actin-dependent protrusion form and position.