Presenting exogenous antigen to T cells.
Presenting exogenous antigen to T cells.
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DOI：
10.1002/0471142735.im1602s88
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发表时间：
2010-02-01
影响因子：
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通讯作者：
Ramachandra, Lakshmi
中科院分区：
文献类型：
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作者：
Harding, Clifford V;Ramachandra, Lakshmi
抗原加工和呈递实验可以用各种各样的抗原呈递细胞（APCs）进行。大多数实验将使用“专业”APC类型之一：树突状细胞（dc）、巨噬细胞和B淋巴细胞。在某些情况下，其他类型的细胞也可用于抗原呈递。每种类型的专业APC都具有重要的抗原呈递功能，但不同类型的APC对免疫反应的贡献不同。因此，选择用于研究的APC类型必须考虑要在实验中建模的免疫反应的阶段或方面。对于某些类型的实验来说，一个重要的技术区别是apc是粘附的还是非粘附的，因为这决定了当介质改变时必须使用的清洗细胞的程序。
Antigen processing and presentation experiments can be done with a wide variety of antigen-presenting cells (APCs). Most experiments will use one of the "professional" APC types: dendritic cells (DCs), macrophages, and B lymphocytes. Other types of cells may be used for antigen presentation in some circumstances. Each type of professional APC has an important antigen-presentation function, but the different APC types contribute to different aspects of the immune response. Therefore, selection of an APC type for study must include consideration of the stage or aspect of immune response that is to be modeled in the experiment. An important technical distinction for some types of experiments is whether the APCs are adherent or nonadherent, since this dictates the procedures that must be used to wash the cells as the medium is changed.