Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines
Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines
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DOI：
10.1186/s12936-016-1254-1
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发表时间：
2016-04-21
期刊：
影响因子：
3
通讯作者：
Clark, Taane G.
中科院分区：
文献类型：
--
作者：
Campino, Susana;Benavente, Ernest Diez;Clark, Taane G.
背景：恶性疟原虫从人类传播到蚊媒需要将在红细胞内复制的无性形式亚群分化为不分裂的雄配子体和雌配子体。疟疾传播所需的这一关键分化事件背后的分子机制的本质尚不完全清楚。方法：使用全基因组测序来检测不产生3D7的配子体衍生品系F12和A4的基因组多样性。结果：从3D7亲本株到其衍生品系F12(培养传代衍生克隆系)和A4(转基因克隆系)的进化发生了变化。结论：为进一步利用这些寄生虫株系研究配子发生或其他表型提供了基因组学资源。
Background: Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.Methods: Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.Results: The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.Conclusion: A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.