A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells.
A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells.
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DOI：
10.1182/blood.v80.7.1725.bloodjournal8071725
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发表时间：
1992-10
期刊：
影响因子：
20.3
通讯作者：
Linda M. Scott;Curt I. Civin;P. Rørth;A. Friedman
中科院分区：
文献类型：
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作者：
Linda M. Scott;Curt I. Civin;P. Rørth;A. Friedman
CCAAT/增强子结合蛋白(C/EBP)家族的成员已被证明调节脂肪细胞和肝细胞的终末分化。在这些细胞谱系中，高水平的C/EBPα只在成熟的、未分裂的细胞中发现。通过Western blotting和免疫组织化学染色，我们确定了C/EBPα、C/EBPβ和C/EBP Delta在骨髓单核细胞分化过程中表达的时间顺序。这些研究表明，C/EBP亚型在髓系中的表达具有独特的时间模式。特别是，C/EBPα在增殖期的骨髓单核细胞中的表达非常高，在表型成熟时表达减弱。虽然我们已经在多发性髓系白血病细胞系中检测到C/EBPα、C/EBPβ和C/EBP Delta，在正常髓系细胞和初发人髓系白血病中也检测到了C/EBPα，但在红系和淋巴系细胞中都没有检测到这些C/EBP亚型。最后，我们发现在成熟的粒细胞中，C/EBPα、C/EBPβ和C/EBP Delta蛋白和信使RNA水平相关。C/EBP同源二聚体和异源二聚体的组织特异性组合的形成可能允许该转录因子家族调节脂肪细胞、肝细胞和骨髓单核细胞中的不同基因集。
Members of the CCAAT/enhancer binding protein (C/EBP) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. In these cell lineages, high levels of C/EBP alpha are found only in mature, nondividing cells. Using Western blotting and immunohistochemical staining, we have determined the temporal order of expression for C/EBP alpha, C/EBP beta, and C/EBP delta in differentiating myelomonocytic marrow cells. These studies show a unique temporal pattern of C/EBP isoform expression in the myeloid lineage. In particular, C/EBP alpha expression is very high in proliferative myelomonocytic cells, and diminishes during phenotypic maturation. While we have detected C/EBP alpha, C/EBP beta, and C/EBP delta in multiple myeloid leukemia cell lines, and C/EBP alpha in normal myeloid cells and in de novo human myeloid leukemias, we have not detected these C/EBP isoforms in either erythroid or lymphoid cells. Finally, we show that C/EBP alpha, C/EBP beta, and C/EBP delta protein and messenger RNA levels correlate in maturing granulocytic cells. The formation of tissue-specific combinations of C/EBP homodimers and heterodimers may allow this family of transcription factors to regulate different sets of genes in adipocytes, hepatocytes, and myelomonocytes.