Genetic characterization of H5N2 influenza viruses isolated form wild birds in Japan suggests multiple reassortment.
Genetic characterization of H5N2 influenza viruses isolated form wild birds in Japan suggests multiple reassortment.
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从日本野生鸟类中分离出的 H5N2 流感病毒的遗传特征表明存在多重重排。
DOI：
10.1007/s00705-016-3023-4
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Imai K.
中科院分区：
文献类型：
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作者：
Sultan S;Bui VN;Hill NJ;Hussein ITM;Trinh DQ;Inage K;Hashizume T;Runstadler JA;Ogawa H;Imai K.
H5亚型的低致病性禽流感病毒（LPAIV）可以突变为高致病性形式，可能破坏家禽业的稳定。野生候鸟被认为是LPAIV的天然宿主，能够传播高致病性和低致病性的病毒。因此，对野生鸟类中AIV的监测和鉴定至关重要。在这里，我们报告了2009年和2011年期间在日本北海道通过监测获得的10株H5N2亚型AIV的分离和遗传特征。全基因组测序结果显示，这些分离株的H5和N2基因均为近缘，属于欧亚禽类样谱系，但与进化枝2.3.4.4的H5高致病性毒株无关。发现分离株的内部基因是多样的，与我们的假设一致，这些H5N2毒株经历了多次重配事件。尽管所有的H5N2分离株的特点是LPAIV的基础上的HA切割位点的氨基酸序列，这种分析表明，一个不同的池的前体，可能种子未来的爆发在家禽和可能的人类传播，这表明需要高质量的监测。
Low-pathogenic avian influenza viruses (LPAIVs) of the H5 subtype can mutate to highly pathogenic forms, potentially destabilizing the poultry industry. Wild migratory birds are considered a natural reservoir of LPAIVs capable of dispersing both high- and low-pathogenic forms of the virus. Therefore, surveillance and characterization of AIV in wild birds are essential. Here, we report on the isolation and genetic characterization of 10 AIVs of the H5N2 subtype obtained through surveillance in Hokkaido, Japan, during 2009 and 2011. Full-genome sequencing revealed that the H5 and N2 genes of these isolates are all closely related to each other, belonging to the Eurasian avian-like lineage, but they are unrelated to H5 highly pathogenic strains of clade 2.3.4.4. The internal genes of the isolates were found to be diverse, consistent with our hypothesis that these H5N2 strains have undergone multiple reassortment events. Even though all of the H5N2 isolates were characterized as LPAIV based on the amino acid sequences at the HA cleavage site, this analysis demonstrates a diverse pool of precursors that may seed future outbreaks in poultry and possible human transmissions, suggesting the need for high-quality surveillance.