High-resolution mass spectrometry of small molecules bound to membrane proteins.
High-resolution mass spectrometry of small molecules bound to membrane proteins.
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DOI：
10.1038/nmeth.3771
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发表时间：
2016-04
期刊：
影响因子：
48
通讯作者：
Robinson CV
中科院分区：
文献类型：
--
作者：
Gault J;Donlan JA;Liko I;Hopper JT;Gupta K;Housden NG;Struwe WB;Marty MT;Mize T;Bechara C;Zhu Y;Wu B;Kleanthous C;Belov M;Damoc E;Makarov A;Robinson CV
已知小分子稳定膜蛋白并调节功能和寡聚状态，但它们的身份通常很难定义。在这里，我们开发和应用高分辨率，轨道阱质谱仪完整的膜蛋白-配体复合物。使用这个平台，我们解决了多个结合事件的复杂性，量化小分子结合，并揭示了内源性脂质的选择性，不同的只是酰基链长度。
Small molecules are known to stabilise membrane proteins and to modulate function and oligomeric state, but their identity is often hard to define. Here we develop and apply a high-resolution, Orbitrap mass spectrometer for intact membrane protein-ligand complexes. Using this platform we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.