Spontaneous mutations of the flavonoid 3′-hydroxylase gene conferring reddish flowers in the three morning glory species
Spontaneous mutations of the flavonoid 3′-hydroxylase gene conferring reddish flowers in the three morning glory species
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DOI：
10.1093/pcp/pcg143
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发表时间：
2003-10-01
影响因子：
4.9
通讯作者：
Iida, S
中科院分区：
文献类型：
--
作者：
Hoshino, A;Morita, Y;Iida, S
在Ipomoea植物中，Ipomoea nil和Ipomoea tricolor都显示明亮的蓝色花朵，Ipomoea purpurea显示深紫色花朵。虽然所有这些花都含有基于花青素的花青素色素，但I。分别携带洋红、粉红和紫红等位基因的L. nil、L. purpurea和L. tricolor产生含有天竺葵素衍生物的红色花，并且它们都缺乏类黄酮3 ′-羟化酶（F3 ′ H）基因。L nil中的洋红等位基因是由单个C至T碱基转换引起的无义突变，产生终止密码子TGA，并且栽培品种Violet携带相同的突变。几个测试的粉红色突变体在I。purpurea在同一位点携带属于Ac/Ds超家族的0.55-kb DNA转座因子Tip 201的插入片段。不能检测到Tip 201从F3 'H基因的切除，并且F3' H转录物的剪接和多腺苷酸化模式都受到Tip 201整合的影响。紫红色等位基因在I. tricolor是产生终止密码子TAG的单个T插入，并且FYH转录物的积累被无义介导的RNA衰变显著降低。自发突变Ipomoea，包括一个可能的创始人突变的粉红色等位基因，也进行了讨论。
Among the Ipomoea plants, both Ipomoea nil and Ipomoea tricolor display bright blue flowers, and Ipomoea purpurea exhibits dark purple flowers. While all of these flowers contain cyanidin-based anthocyanin pigments, the mutants of I. nil, L purpurea, and L tricolor carrying the magenta, pink, and fuchsia alleles, respectively, produce reddish flowers containing pelargonidin derivatives, and all of them are deficient in the gene for flavonoid 3'-hydroxylase (F3'H). The magenta allele in L nil is a nonsense mutation caused by a single C to T base transition generating the stop codon TGA, and the cultivar Violet carries the same mutation. Several tested pink mutants in I. purpurea carry inserts of the 0.55-kb DNA transposable element Tip201 belonging to the Ac/Ds superfamily at the identical site. No excision of Tip201 from the F3'H gene could be detected, and both splicing and polyadenylation patterns of the F3'H transcripts were affected by the Tip201 integration. The fuchsia allele in I. tricolor is a single T insertion generating the stop codon TAG, and the accumulation of the FYH transcripts was drastically reduced by the nonsense-mediated RNA decay. Spontaneous mutations in Ipomoea, including a possible founder mutation in the pink allele, are also discussed.