The expression of X-linked phosphoglycerate kinase in the early mouse embryo.
The expression of X-linked phosphoglycerate kinase in the early mouse embryo.
复制标题
X连锁磷酸甘油酸激酶在早期小鼠胚胎中的表达。
DOI：
10.1111/j.1432-0436.1982.tb01276.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
K. Illmensee
中科院分区：
文献类型：
--
作者：
W. Krietsch;R. Fundele;G. Kuntz;M. Fehlau;K. Bürki;K. Illmensee
在早期小鼠胚胎发生，X染色体连锁的母亲和父亲的磷酸甘油酸激酶（PGK-1）等位基因的活性测定，使用电泳分离的基因产物和灵敏的荧光酶测定。在从与PGK-1a雄性交配的PGK-1b纯合子雌性收集的胚胎中，反之亦然，在植入后第6天首次检测到父系来源的等位酶。在PGK-1b和PGK-1a杂合子的雌性胚胎中研究了母系遗传等位基因的表达。从第1天到第4天，胚胎保持PGK-1B和PGK-1A酶活性的恒定比例。在第4天和第5天之间植入胚胎之前，由于首次出现了源自母系遗传等位基因的新合成PGK，两种PGK-1等位基因变体的活性比率发生了显著变化。我们的数据表明，PGK合成的发病时间的差异取决于这个特定的基因产物是母亲或父亲的起源。因此，我们得出结论，母体PGK-1基因座已经激活在植入前发育后期，而父系遗传的基因座保持沉默，在植入前阶段，但随后在大约X染色体失活的时间表达。
During early mouse embryogenesis, the activity of X-chromosomally linked maternal and paternal phosphoglycerate kinase (PGK-1) alleles was determined using electrophoretic separation of their gene products and a sensitive fluorometric enzyme assay. In the embryos collected from females homozygous for PGK-1b mated with PGK-1a males and vice versa, the paternally derived allozyme was first detected after implantation on day 6. Expression of the maternally inherited allele was studied in embryos from females heterozygous for PGK-1b and PGK-1a. From day 1 to day 4, the embryos maintained a constant ratio of enzyme activity of PGK-1B to PGK-1A. Prior to implantation of the embryos between day 4 and day 5, the activity ratio of the two PGK-1 allelic variants changed significantly due to the first appearance of newly synthesized PGK derived from the maternally inherited allele. Our data demonstrate a temporal difference in the onset of PGK synthesis depending on whether this particular gene product is of maternal or paternal origin. Therefore, we conclude that the maternal PGK-1 locus is already activated during late preimplantation development whereas the paternally inherited gene locus remains silent at the preimplantation stage but is subsequently expressed at approximately the time of X-chromosomal inactivation.